• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Order
Home >> Antibodies >> Recombinant Vimentin Antibody

Recombinant Vimentin Antibody [clone VIM/1937R] (V8356)

NSJ bio Image Pdf Image
  Catalog No Formulation Size Price (USD)  
Image V8356-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
Image
V8356-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
Image
V8356SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
Bulk quote request
IHC staining of FFPE human tonsil with recombinant Vimentin antibody (clone VIM/1937R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
›
IHC staining of FFPE human tonsil with recombinant Vimentin antibody (clone VIM/1937R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon carcinoma with recombinant Vimentin antibody (clone VIM/1937R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human melanoma with recombinant Vimentin antibody (clone VIM/1937R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human A375 and U-87 cell lysate with Vimentin antibody (clone VIM/1937R). Expected molecular weight: 53-58 kDa.
SDS-PAGE analysis of purified, BSA-free Vimentin antibody (clone VIM/1937R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name VIM/1937R
Purity Protein A affinity chromatography
UniProt P08670
Localization Cytoplasmic
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant Vimentin antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Related Products

  • Applications : WB, IHC
    Reactivity : Human
    Recrabbitmono
  • Applications : FACS, WB, IHC-P
    Reactivity : Human
    Microvalidated
  • Applications : FACS
    Reactivity : Human
    Microvalidated
  • Applications : WB, IHC-P, IF
    Reactivity : Human, Rabbit, Pig, Rat
  • Applications : IHC-P
    Reactivity : Human, Mouse, Rat
    Citation  Citations(11)
  • Applications : WB, IHC-P
    Reactivity : Human
    Citation  Citations(1)
  • Applications : WB, IHC-P
    Reactivity : Human
  • Applications : WB, IHC-P
    Reactivity : Human
  • Applications : WB, ICC/IF, IP
    Reactivity : Human
  • Applications : IHC-P, WB, IF
    Reactivity : Human
    Rab Mono Image
  • Applications : WB, IHC-P
    Reactivity : Human
  • Applications : WB, IHC-P, IF
    Reactivity : Human, Mouse, Rat
  • Applications : IF, WB, IHC, ELISA
    Reactivity : Human, Mouse
    Pred. Reactivity : Bovine, Hamster, Primate, Rat
  • Applications : IF, WB, IHC, FACS, ELISA
    Reactivity : Human
    Pred. Reactivity : Bovine, Chicken, Hamster, Mouse, Pig, Primate, Rat, Xenopus
  • Applications : WB, IHC, FACS, ELISA
    Reactivity : Human
    Pred. Reactivity : Mouse, Rat, Bovine, Pig, Hamster
  • Applications : IF, WB, IHC, FACS, ELISA
    Reactivity : Human
  • Applications : WB, ELISA (peptide)
    Reactivity : Human, Mouse, Rat
    Pred. Reactivity : Dog, Pig, Cow

Description

This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

Application Notes

Optimal dilution of the recombinant Vimentin antibody should be determined by the researcher.

Immunogen

Full length recombinant protein was used as the immunogen for this recombinant Vimentin antibody.

Storage

Store the recombinant Vimentin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.