- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
TUBB3 Antibody / Neuronal Marker Antibody [clone rTUBB3/7405] (V4451)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V4451-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 ug | 559 | |
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V4451-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 20 ug | 259 | |
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V4451SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human, Mouse, Rat, Hamster, Rabbit, Guinea pig |
| Format | Purified |
| Host | Mouse |
| Clonality | Recombinant Mouse Monoclonal |
| Isotype | Mouse IgG1, kappa |
| Clone Name | rTUBB3/7405 |
| Purity | Protein A/G affinity |
| UniProt | Q13509 |
| Localization | Cytoplasm |
| Applications | Western Blot : 2-4ug/ml (Human/Mouse/Rat/Hamster/Rabbit/Guinea pig) Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT (Human) |
| Limitations | This TUBB3 Antibody / Neuronal Marker Antibody is available for research use only. |
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Tubulin beta 3 class III (TUBB3), also known as Beta III tubulin, is a neuron-enriched microtubule protein that serves as a key structural component of the neuronal cytoskeleton. TUBB3 is predominantly localized in the cytoplasm of neurons, where it integrates into microtubules that support axonal transport, neurite extension, and maintenance of neuronal morphology. The TUBB3 Antibody / Neuronal Marker Antibody is designed to detect this highly specific cytoskeletal protein, enabling reliable identification of neuronal populations in both biochemical and tissue-based analyses.
TUBB3 antibody, also referred to as Beta III tubulin antibody and TUJ1 antibody in the literature, recognizes a protein that is strongly enriched in neurons of the central and peripheral nervous systems, with minimal expression in most non-neuronal cell types. This restricted expression profile makes TUBB3 one of the most widely used markers of neuronal differentiation and lineage. Western blot analysis demonstrates a clear and consistent band at approximately 50â55 kDa across brain lysates from multiple species, including human, mouse, rat, hamster, guinea pig, and rabbit, consistent with the predicted molecular weight of TUBB3. The uniform detection across species highlights the high degree of conservation of this protein and supports its use in cross-species neural studies.
Structurally, TUBB3 forms heterodimers with alpha-tubulin to assemble into microtubules, which are essential for intracellular transport, cell polarity, and structural integrity. The Beta III isoform is distinguished from other tubulin isoforms by its neuron-specific expression pattern and its role in regulating microtubule dynamics. Post-translational modifications such as acetylation, detyrosination, and polyglutamylation further modulate microtubule stability and function, contributing to the specialized properties of neuronal microtubules.
Functionally, TUBB3 plays a critical role in neuronal development, including axon guidance, neuronal migration, and synaptic formation. Its expression is upregulated during neuronal differentiation and remains elevated in mature neurons, reflecting its importance in maintaining neuronal architecture and function. In brain tissue, TUBB3 is prominently expressed in neuronal cell bodies and processes, providing a robust marker for visualizing neuronal networks and organization.
In addition to its role in normal neural physiology, TUBB3 has been implicated in cancer biology, particularly in tumors with neural or neuroendocrine differentiation. Elevated TUBB3 expression has been associated with altered microtubule dynamics and resistance to microtubule-targeting chemotherapeutic agents, making it a relevant marker in studies of tumor biology and therapeutic response. Its expression in tumor cells can provide insight into lineage identity and differentiation status.
Immunohistochemical analysis of human brain tissue demonstrates strong cytoplasmic staining in neurons and neuropil, highlighting the extensive microtubule network within neural tissue. This characteristic staining pattern provides clear visualization of neuronal morphology and supports the use of this antibody as a reliable neuronal marker. Clone rTUBB3/7405 is a recombinant mouse monoclonal antibody designed to detect TUBB3 with high specificity, offering consistent performance in western blot and tissue-based applications and serving as a robust tool for studies of neuronal biology, cytoskeletal organization, and neural tissue structure across species.
This antibody is part of a broader antibody panel offered by NSJ Bioreagents.
Optimal dilution of the TUBB3 Antibody / Neuronal Marker Antibody should be determined by the researcher.
A recombinant partial protein sequence (within amino acids 250-450) from the human protein was used as the immunogen for the recombinant TUBB3 antibody.
Aliquot the TUBB3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
TUBB3 antibody, Beta III tubulin antibody, TUJ1 antibody, TUBB3 neuronal marker antibody, Beta III tubulin IHC antibody
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