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Home >> Antibodies >> Recombinant TTF-1 Antibody / NKX2.1

Recombinant TTF-1 Antibody / NKX2.1 [clone rNX2.1/690] (V3654)

  Catalog No Formulation Size Price (USD)  
V3654-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 379

V3654-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199

V3654SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 399

V3654IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 379
IHC: Formalin-fixed, paraffin-embedded human thyroid stained with recombinant TTF-1 antibody (clone rNX2.1/690). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC: Formalin-fixed, paraffin-embedded human lung adenocarcinoma stained with recombinant TTF-1 antibody (clone rNX2.1/690). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free recombinant TTF-1 antibody (clone rNX2.1/690) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human, Mouse, and Rat. Other species not known.
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rNX2.1/690
Purity Protein G affinity chromatography
UniProt P43699
Localization Nuclear
Applications Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This recombinant TTF-1 antibody is available for research use only.
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Description

Recognizes a protein of 40kDa, identified as Thyroid transcription factor-1, also called NKX2.1. TTF-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. Anti-TTF-1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates. Loss of TTF-1 expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. TTF-1 reactivity is also seen in thyroid malignancies.

Application Notes

Optimal dilution of the recombinant TTF-1 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant protein was used as the immunogen for the recombinant TTF-1 antibody.

Storage

Store the recombinant TTF-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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