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Home >> Antibodies >> Recombinant TRAcP Antibody / TRAP / ACP5

Recombinant TRAcP Antibody / TRAP / ACP5 [clone ACP5/2336R] (V3534)

  Catalog No Formulation Size Price (USD)  
Image V3534-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 459
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V3534-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 209
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V3534SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 459
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V3534IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 459
IHC testing of FFPE human spleen with recombinant TRAcP antibody (clone ACP5/2336R). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free recombinant TRAcP antibody (clone ACP5/2336R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name ACP5/2336R
Purity Protein A affinity chromatography
UniProt P13686
Localization Cytoplasmic (lysosomes)
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This recombinant TRAcP antibody is available for research use only.
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Description

It recognizes a protein of 35kDa, which is identified as tartrate-resistant acid phosphatase (TRAcP). It exists as two isoforms (5a and 5b). This MAb reacts with both the isoforms. Serum TRAcP 5a is secreted by macrophages and dendritic cells and increased in many patients of rheumatoid arthritis. Serum TRAcP 5b is produced from osteoclasts and elevated during bone resorption. TRAcP is an iron containing glycoprotein, which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. TRAcP is synthesized as a latent proenzyme and is activated by proteolytic cleavage and reduction. Normally, TRAcP is highly expressed by osteoclasts, activated macrophages, neurons and endometrium during pregnancy. Expression of TRAcP is increased in certain pathological conditions such as Leukemic Reticuloendotheliosis (Hairy Cell Leukemia), Gaucher’s Disease, HIV-induced Encephalopathy, Osteoclastoma and in osteoporosis and metabolic bone diseases. Anti-TRAcP antibody labels the cells of Hairy Cell Leukemia (HCL) with a high degree of sensitivity and specificity. Other cells stained with this antibody are tissue macrophages and osteoclasts.

Application Notes

Optimal dilution of the recombinant TRAcP antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant full-length human protein was used as the immunogen for the recombinant TRAcP antibody.

Storage

Store the recombinant TRAcP antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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