- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
TP53 Antibody / Cell Cycle Checkpoint Protein Antibody [clone rTP53/8063] (V4455)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V4455-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 ug | 559 | |
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V4455-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 20 ug | 259 | |
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V4455SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
|
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Mouse |
| Clonality | Recombinant Mouse Monoclonal |
| Isotype | Mouse IgG1, kappa |
| Clone Name | rTP53/8063 |
| Purity | Protein A/G affinity |
| UniProt | P04637 |
| Localization | Nucleus |
| Applications | Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT |
| Limitations | This TP53 antibody is available for research use only. |
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Tumor protein p53 (TP53) is a nuclear transcription factor that functions as a central cell cycle checkpoint protein in mammalian cells. Acting as a genomic surveillance factor, p53 monitors DNA integrity and activates transcriptional programs that halt cell cycle progression when genomic damage is detected. The TP53 Antibody / Cell Cycle Checkpoint Protein Antibody detects this key cell cycle checkpoint protein, widely known as p53, which enforces checkpoint control at critical stages of the cell cycle to prevent replication of damaged DNA.
TP53 antibody, also referred to as p53 antibody or Tumor protein p53 antibody in the literature, targets a protein that serves as one of the most important cell cycle checkpoint proteins in the DNA damage response network. Under normal physiological conditions the cell cycle checkpoint protein p53 is maintained at low levels through continuous ubiquitin-mediated degradation primarily driven by the E3 ubiquitin ligase MDM2. When cells encounter genotoxic stress such as DNA damage, oncogene activation, replication errors, or oxidative stress, stabilization of the cell cycle checkpoint protein allows rapid nuclear accumulation where it initiates checkpoint signaling pathways.
Once activated, the cell cycle checkpoint protein p53 functions as a sequence-specific transcription factor that regulates genes responsible for checkpoint enforcement. One of the most important transcriptional targets of the cell cycle checkpoint protein is CDKN1A (p21), a cyclin-dependent kinase inhibitor that blocks cyclin-CDK complexes and prevents progression through the G1/S and G2/M cell cycle transitions. Through induction of p21 and related regulators, the cell cycle checkpoint protein p53 establishes protective arrest points that allow cells time to repair damaged DNA before replication or mitosis proceeds.
These checkpoint mechanisms play a critical role in maintaining genomic stability by preventing replication of damaged DNA and limiting propagation of mutations. If genomic damage persists despite checkpoint activation, the cell cycle checkpoint protein p53 can redirect cellular signaling toward apoptosis through induction of pro-apoptotic genes such as BAX, PUMA, and NOXA. In this way p53 integrates checkpoint control with apoptosis pathways to eliminate genetically compromised cells.
Loss of TP53 cell cycle checkpoint protein function is one of the most frequent molecular events in human cancer. Mutations affecting the TP53 gene disrupt checkpoint signaling pathways and allow cells to bypass cell cycle arrest despite genomic damage. In many tumors mutant forms of the checkpoint protein accumulate within the nucleus due to impaired degradation mechanisms, producing elevated p53 protein levels that are frequently detected in cancer tissues.
A recombinant mouse monoclonal TP53 antibody such as clone rTP53/8063 is suitable for detecting the p53 cell cycle checkpoint protein in studies examining checkpoint signaling networks, cell cycle regulation mechanisms, and molecular pathways that maintain genomic integrity.
Optimal dilution of the TP53 Antibody / Cell Cycle Checkpoint Protein Antibody should be determined by the researcher.
Recombinant full-length human protein was used as the immunogen for the TP53 Antibody / Cell Cycle Checkpoint Protein Antibody.
Aliquot the recombinant TP53 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
p53 antibody, Tumor protein p53 antibody, Cellular tumor antigen p53 antibody, Phosphoprotein p53 antibody, Transformation related protein 53 antibody
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