Topoisomerase II alpha Antibody / Mitotic Chromosome Enzyme Antibody [clone TOP2A/6570R] (V9310)

Topoisomerase II alpha Antibody / Mitotic Chromosome Enzyme Antibody (clone TOP2A/6570R). Immunohistochemistry analysis of FFPE human lymph node tissue demonstrates strong nuclear staining of DNA Topoisomerase II alpha in proliferating lymphoid cells. Nuclear immunoreactivity is enriched within germinal center lymphocytes, reflecting the high mitotic activity of these cells where mitotic chromosome condensation and structural organization are actively occurring. As a Mitotic Chromosome Enzyme Antibody, clone TOP2A/6570R highlights cells undergoing mitosis in which DNA Topoisomerase II alpha supports chromosome condensation and mitotic chromosome architecture required for accurate cell division. HIER: boil tissue sections in pH 9 Tris-EDTA buffer for 20 min followed by cooling at RT prior to immunohistochemistry staining.

Topoisomerase II alpha Antibody / Mitotic Chromosome Enzyme Antibody (clone TOP2A/6570R). Immunohistochemistry analysis of FFPE human tonsil tissue demonstrates strong nuclear staining of DNA Topoisomerase II alpha in proliferating lymphoid cells. Nuclear immunoreactivity is most prominent within germinal center lymphocytes, reflecting the elevated mitotic activity of these cells where mitotic chromosome condensation and organization are actively occurring. As a Mitotic Chromosome Enzyme Antibody, clone TOP2A/6570R highlights cells undergoing mitosis in which DNA Topoisomerase II alpha supports mitotic chromosome architecture and chromosome condensation required for accurate cell division. HIER: boil tissue sections in pH 9 Tris-EDTA buffer for 20 min followed by cooling at RT prior to immunohistochemistry staining.

Negative control: IHC staining of FFPE human brain tissue using recombinant Topoisomerase II alpha antibody (clone TOP2A/6570R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Topoisomerase II alpha Antibody / Mitotic Chromosome Enzyme Antibody (clone TOP2A/6570R). Western blot analysis of human cell lysates demonstrates detection of DNA Topoisomerase II alpha following SDS-PAGE separation and membrane transfer. Lane 1: HeLa cell lysate. Lane 2: Jurkat cell lysate. A band is detected at approximately 170-180 kDa, consistent with the predicted molecular weight of DNA Topoisomerase II alpha / TOP2A (~174 kDa). The detected protein corresponds to the mitotic chromosome enzyme responsible for regulating DNA topology during chromosome condensation and mitotic chromosome organization. Detection of the denatured TOP2A protein band supports the use of this Mitotic Chromosome Enzyme Antibody for western blot analysis of this mitotic chromosome regulatory enzyme in human cell lysates.

SDS-PAGE analysis of purified, BSA-free recombinant Topoisomerase II alpha antibody (clone TOP2A/6570R) as confirmation of integrity and purity.