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Home >> Antibodies >> Recombinant TLE1 Antibody / Transducin-like enhancer protein 1

Recombinant TLE1 Antibody / Transducin-like enhancer protein 1 [clone TLE1/2946R] (V7357)

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  Catalog No Formulation Size Price (USD)  
Image V7357-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7357-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7357SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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IHC testing of FFPE human endometrial carcinoma with recombinant TLE1 antibody (clone TLE1/2946R). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
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IHC testing of FFPE human endometrial carcinoma with recombinant TLE1 antibody (clone TLE1/2946R). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant TLE1 antibody (clone TLE1/2946R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name TLE1/2946R
Purity Protein A affinity chromatography
UniProt Q04724
Localization Nuclear
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This recombinant TLE1 antibody is available for research use only.
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Description

Key players in the Notch pathway are the TLE genes, which are human homologs of the Drosophila groucho gene. Groucho is a transcriptional repressor that plays a key role in neurogenesis, segmentation and sex determination. Transducin-like enhancer protein 1 (TLE1) is a protein that is encoded by the TLE1 gene and is involved in control of hematopoiesis, neuronal, and terminal epithelial differentiation. Positive immunohistochemical nuclear staining with anti-TLE-1 has been shown to be a useful addition to an IHC panel when differentiating synovial sarcoma from other soft tissue malignancies.

Application Notes

Optimal dilution of the recombinant TLE1 antibody should be determined by the researcher.

Immunogen

A portion of amino acids 175-338 from the human protein was used as the immunogen for the recombinant TLE1 antibody.

Storage

Store the recombinant TLE1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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