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Home >> Antibodies >> TG Antibody Recombinant Mouse MAb r6E1 / Thyroglobulin Antibody

TG Antibody Recombinant Mouse MAb r6E1 / Thyroglobulin Antibody [clone r6E1] (V3629)

  Catalog No Formulation Size Price (USD)  
Image V3629-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 559
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V3629-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 259
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V3629SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
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V3629IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 559
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TG Antibody Recombinant Mouse MAb r6E1. Immunohistochemistry analysis of Thyroglobulin using TG Antibody Recombinant Mouse MAb r6E1 in FFPE human thyroid gland tissue section. HRP-DAB brown chromogenic staining highlights strong cytoplasmic signal in thyroid follicular epithelial cells forming thyroid follicles, with prominent staining of follicular luminal colloid material. The staining pattern is characteristic of Thyroglobulin expression in normal thyroid follicular epithelium and reflects its established role as a marker of thyroid follicular cell differentiation.
TG Antibody Recombinant Mouse MAb r6E1. Immunohistochemistry analysis of Thyroglobulin using TG Antibody Recombinant Mouse MAb r6E1 in FFPE human thyroid gland tissue section. HRP-DAB brown chromogenic staining highlights cytoplasmic signal in thyroid follicular epithelial cells forming well defined thyroid follicles, with strong staining of follicular luminal colloid material. The staining pattern is characteristic of Thyroglobulin expression in normal thyroid follicular epithelium and supports its role as a marker of thyroid follicular cell differentiation.
SDS-PAGE analysis of purified, BSA-free recombinant TG antibody (clone r6E1) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Host Mouse
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name r6E1
Purity Protein G affinity chromatography
UniProt P01266
Localization Cytoplasmic, secreted
Applications Immunohistochemistry (FFPE) : 0.25-0.5ug/ml for 30 min at RT
Limitations This recombinant TG antibody is available for research use only.
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Description

Thyroglobulin is a 660 kDa dimeric pre-protein with mutiple glycosylation sites. It is produced by and processed within the thyroid gland to produce the hormone thyroxine and triiodothyronine. Prior to forming dimers, thyroglobulin monomers undergo conformational maturation in the endoplasmic reticulation. The vast majority of follicular carcinomas of the thyroid will give positive immunoreactivity for anti-thyroglobulin even though sometimes only focally. Poorly differentiated carcinomas of the thyroid are frequently anti-thyroglobulin negative. Adenocarcinomas of other-than-thyroid origin do not react with this antibody. This antibody is useful in identification of thyroid carcinoma of the papillary and follicular types. Presence of thyroglobulin in metastatic lesions establishes the thyroid origin of tumor. Anti-thyroglobulin, combined with anti-calcitonin, can identify medullary carcinomas of the thyroid. Furthermore, anti-thyroglobulin, combined with anti-TTF1, can be a reliable marker to differentiate between primary thyroid and lung neoplasms.

Application Notes

Optimal dilution of the TG Antibody Recombinant Mouse MAb r6E1 should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Human thyroid follicular cells were used as the immunogen for the TG Antibody Recombinant Mouse MAb r6E1.

Storage

Store the recombinant TG antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

Thyroglobulin antibody, TG protein antibody, Thyroid hormone precursor protein antibody, Thyroid follicular cell marker antibody

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