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Home >> Antibodies >> Recombinant TdT Antibody / DNA nucleotidylexotransferase

Recombinant TdT Antibody / DNA nucleotidylexotransferase [clone DNTT/4617R] (V8860)

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  Catalog No Formulation Size Price (USD)  
Image V8860-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V8860-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V8860SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human thymoma with recombinant TdT antibody (clone DNTT/4617R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human thymoma with recombinant TdT antibody (clone DNTT/4617R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human thymoma tissue with recombinant TdT antibody (clone DNTT/4617R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human thymus tissue with recombinant TdT antibody (clone DNTT/4617R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant TdT antibody (clone DNTT/4617R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name DNTT/4617R
Purity Protein A/G affinity
UniProt P04053
Localization Nucleus
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant TdT antibody is available for research use only.
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Description

Terminal deoxynucleotidyl transferase (TdT) is an unusual deoxynucleotide polymerizing enzyme with a molecular weight of about 58 kDa found normally only in B- and T-cell lymphoblasts/prelymphocytes. TdT generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Rare TdT-positive cells are regularly detected in thymus and bone marrow. Typically, TdT expression in the thymus is very variable from cell to cell since it is rapidly decreased in more mature T-cells. TdT-positive cells may occasionally be found in tonsils, lymph nodes and extranodal lymphoid tissue. Immunohistochemical detection of TdT has value in classification of malignant lymphomas and acute leukemias, particularly for the identification of pre-B and pre-T acute lymphoblastic leukemia/lymphoblastic lymphoma (ALL/LBL).

Application Notes

Optimal dilution of the recombinant TdT antibody should be determined by the researcher.

Immunogen

A portion of amino acids 52-192 was used as the immunogen for the recombinant TdT antibody.

Storage

Aliquot the recombinant TdT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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