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Home >> Antibodies >> Recombinant TAG-72 Antibody

Recombinant TAG-72 Antibody [clone rB72.3] (V7718)

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  Catalog No Formulation Size Price (USD)  
Image V7718-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7718-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7718SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC staining of human breast carcinoma with recombinant TAG-72 antibody (clone rB72.3). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
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IHC staining of human breast carcinoma with recombinant TAG-72 antibody (clone rB72.3). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant TAG-72 antibody (clone rB72.3) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rB72.3
Purity Protein G affinity chromatography
Gene ID 182875
Localization Cytoplasmic and cell surface
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant TAG-72 antibody is available for research use only.
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Description

This antibody recognizes an oncofetal antigen of 220kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This antibody defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80% sensitivity and 93% specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of TAG-72 Ab with a GCDFP-15 Ab is valuable in the diagnosis of apocrine carcinoma.

Application Notes

Optimal dilution of the recombinant TAG-72 antibody should be determined by the researcher.

Immunogen

The membrane-enriched fraction of a human breast carcinoma liver metastasis was used as the immunogen for this recombinant TAG-72 antibody.

Storage

Store the recombinant TAG-72 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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