Succinate Dehydrogenase B Antibody | Recombinant SDHB

	Catalog No	Formulation	Size	Price (USD)
	V4680-100UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide	100 ug	559
Formulation
	V4680-20UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide	20 ug	259
Formulation
	V4680SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	559
Formulation

Succinate Dehydrogenase B Antibody Human HepG2 WB. Western blot testing of human HepG2 cell lysate was performed with Succinate Dehydrogenase B Antibody. A strong specific band is detected at approximately 29 kDa, within the predicted molecular weight range of 25-32 kDa for SDHB. This Succinate Dehydrogenase B Antibody demonstrates detection of the mitochondrial Complex II iron-sulfur subunit in human HepG2 cells.

Succinate Dehydrogenase B Antibody Human Hepatocellular Carcinoma IHC. IHC staining of FFPE human hepatocellular carcinoma tissue was performed with Succinate Dehydrogenase B Antibody, clone SDHB/8974R. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9.0, 10 mM Tris with 1 mM EDTA for 20 minutes, followed by cooling prior to staining. Strong cytoplasmic SDHB staining is observed throughout the tumor cells. This Succinate Dehydrogenase B Antibody demonstrates localization of the mitochondrial Complex II iron-sulfur subunit in human hepatocellular carcinoma tissue.

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Succinate Dehydrogenase B Antibody Human HepG2 WB. Western blot testing of human HepG2 cell lysate was performed with Succinate Dehydrogenase B Antibody. A strong specific band is detected at approximately 29 kDa, within the predicted molecular weight range of 25-32 kDa for SDHB. This Succinate Dehydrogenase B Antibody demonstrates detection of the mitochondrial Complex II iron-sulfur subunit in human HepG2 cells.

Succinate Dehydrogenase B Antibody Human Hepatocellular Carcinoma IHC. IHC staining of FFPE human hepatocellular carcinoma tissue was performed with Succinate Dehydrogenase B Antibody, clone SDHB/8974R. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9.0, 10 mM Tris with 1 mM EDTA for 20 minutes, followed by cooling prior to staining. Strong cytoplasmic SDHB staining is observed throughout the tumor cells. This Succinate Dehydrogenase B Antibody demonstrates localization of the mitochondrial Complex II iron-sulfur subunit in human hepatocellular carcinoma tissue.

Availability	1-3 business days
Species Reactivity	Human
Format	Purified
Host	Rabbit
Clonality	Recombinant Rabbit Monoclonal
Isotype	Rabbit IgG, kappa
Clone Name	SDHB/8974R
Purity	Protein A/G affinity
UniProt	P21912
Localization	Cytoplasm (Mitochondria)
Applications	Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT Western Blot : 2-4ug/ml
Limitations	This Succinate Dehydrogenase B Antibody / SDHB Recombinant Antibody is available for research use only.
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Description

Succinate Dehydrogenase B Antibody / SDHB Recombinant Antibody detects SDHB, the iron-sulfur subunit of mitochondrial succinate dehydrogenase, also known as respiratory Complex II. SDHB is encoded by the nuclear SDHB gene and functions together with SDHA, SDHC and SDHD to form the succinate dehydrogenase complex within the inner mitochondrial membrane. Complex II has a distinctive metabolic role because it participates in both the tricarboxylic acid (TCA) cycle and the mitochondrial electron transport chain.

During the TCA cycle, succinate dehydrogenase catalyzes the oxidation of succinate to fumarate. Electrons generated during this reaction are transferred from the flavin-containing SDHA subunit through a series of iron-sulfur clusters located within SDHB. They are subsequently transferred toward ubiquinone through the membrane-associated components of the complex. This process connects TCA cycle metabolism with mitochondrial respiration and contributes to efficient cellular energy production.

SDHB is particularly important for the electron-transfer function of Complex II. Disruption of SDHB or other succinate dehydrogenase components can impair mitochondrial respiration and result in intracellular accumulation of succinate. Elevated succinate can influence cellular signaling and gene regulation through inhibition of alpha-ketoglutarate-dependent enzymes, linking mitochondrial metabolic dysfunction with broader changes in cellular physiology.

Alterations affecting SDHB are extensively studied in cancer biology. Germline SDHB mutations are associated with hereditary paraganglioma and pheochromocytoma syndromes, and disruption of succinate dehydrogenase function has also been investigated in other tumor types. Succinate accumulation resulting from SDH deficiency can contribute to altered oxygen-sensing and epigenetic pathways, supporting the characterization of succinate as an oncometabolite in SDH-deficient disease.

Clone SDHB/8974R is a recombinant rabbit monoclonal antibody developed for recognition of SDHB. Analysis of SDHB protein expression can support research into mitochondrial Complex II function, oxidative metabolism and SDH-associated tumor biology. SDHB is also widely investigated in studies of mitochondrial dysfunction, oxidative stress and cellular energy metabolism.

NSJ Bioreagents offers clone SDHB/8974R for research into succinate dehydrogenase expression and mitochondrial biology. This recombinant antibody provides a defined reagent for investigating SDHB in experimental samples and studying the relationship between Complex II function, cellular metabolism and disease.

Researchers studying succinate dehydrogenase expression and mitochondrial Complex II function can explore our SDHB Antibody page for additional reagents targeting the Complex II iron-sulfur subunit.

Application Notes

Optimal dilution of the Succinate Dehydrogenase B Antibody / SDHB Recombinant Antibody should be determined by the researcher.

Immunogen

A synthetic peptide corresponding to human SDHB protein was used as the immunogen for the Succinate dehydrogenase B antibody.

Storage

Aliquot the Succinate dehydrogenase B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Alternate Names

Succinate Dehydrogenase B antibody, SDHB antibody, SDHB Recombinant antibody, Succinate Dehydrogenase Subunit B antibody, Succinate Dehydrogenase Iron-Sulfur Subunit antibody, Complex II Iron-Sulfur Subunit antibody, SDH2 antibody

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Succinate Dehydrogenase B Antibody / SDHB Recombinant Antibody [clone SDHB/8974R] (V4680)

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