- Tel: 858.663.9055
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Recombinant Spectrin beta III Antibody / SPTBN2 [clone SPTBN2/2887R] (V7355)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
 |
V7355-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 519 | |
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V7355-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 229 | |
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V7355SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 519 |
| Species Reactivity | Human |
| Format | Purified |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG, kappa |
| Clone Name | SPTBN2/2887R |
| Purity | Protein A affinity chromatography |
| UniProt | O15020 |
| Localization | Cell surface, cytoplasm |
| Applications | Flow cytometry : 1-2ug/10^6 cells Immunofluorescence : 1-2ug/ml Western blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT |
| Limitations | This recombinant Spectrin beta III antibody is available for research use only. |
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Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II), four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin and spectrin are present in erythrocytes, whereas spectrin II (also designated fodrin ) and spectrin I (also designated fodrin ) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Spectrin III is highly homologous to both spectrin I and spectrin II. Spectrin III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta.
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant Spectrin beta III antibody to be titered up or down for optimal performance.
Human recombinant partial protein corresponding to amino acids 356-475 was used as the immunogen for this recombinant Spectrin beta III antibody.
Store the recombinant Spectrin beta III antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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