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Home >> Antibodies >> Recombinant Spectrin alpha 1 Antibody / SPTA1

Recombinant Spectrin alpha 1 Antibody / SPTA1 [clone SPTA1/2939R] (V7343)

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  Catalog No Formulation Size Price (USD)  
Image V7343-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7343-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7343SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V7343IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
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IHC staining of FFPE human placenta with recombinant Spectrin alpha 1 antibody (clone SPTA1/2939R). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
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IHC staining of FFPE human placenta with recombinant Spectrin alpha 1 antibody (clone SPTA1/2939R). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE human placenta with recombinant Spectrin alpha 1 antibody (clone SPTA1/2939R). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free recombinant Spectrin alpha 1 antibody (clone SPTA1/2939R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name SPTA1/2939R
Purity Protein A affinity chromatography
UniProt P02549
Localization Cytoplasmic, cell surface
Applications ELISA : 2-4ug/ml (order BSA/azide-free format)
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This recombinant Spectrin alpha 1 antibody is available for research use only.
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Description

Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. Spectrins function as membrane organizers and stabilizers, composed of non-homologous and chains, which aggregate side-to-side in an anti-parallel fashion to form dimers, tetramers and higher polymers. Spectrin Ia and spectrin Ib are present in erythrocytes, whereas spectrin IIa (also designated fodrin alpha) and spectrin IIb (also designated fodrin beta) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function.

Application Notes

Optimal dilution of the recombinant Spectrin alpha 1 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A portion of amino acids 356-475 from the human protein was used as the immunogen for the recombinant Spectrin alpha 1 antibody.

Storage

Store the recombinant Spectrin alpha 1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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