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Home >> Antibodies >> Recombinant SOX2 Antibody

Recombinant SOX2 Antibody [clone SBX2-1R] (V7640)

  Catalog No Formulation Size Price (USD)  
Image V7640-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7640-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7640SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human cervical carcinoma with recombinant SOX2 antibody (clone SBX2-1R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Western blot testing of NCCIT lysate with recombinant SOX2 antibody (clone SBX2-1R). Predicted molecular weight ~34 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name SBX2-1R
Purity Protein A affinity chromatography
UniProt P48431
Localization Nuclear
Applications ELISA (order BSA-free format for coating) :
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant SOX2 antibody is available for research use only.
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Description

Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. [UniProt]

Application Notes

The stated application concentrations are suggested starting points. Titration of the SOX2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Amino acids 176-305 were used as the immunogen for the recombinant SOX2 antibody.

Storage

Store the recombinant SOX2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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