SOX10 Antibody rSOX10/1074 / SRY-Box Transcription Factor 10 Antibody [clone rSOX10/1074] (V3563)

SOX10 Antibody rSOX10/1074 by IHC in Human Melanoma Tissue. Immunohistochemistry staining of FFPE human melanoma tissue using recombinant mouse monoclonal SOX10 antibody rSOX10/1074. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM citrate buffer at pH 6 for 10-20 minutes, followed by cooling at room temperature for 20 minutes. Strong brown nuclear staining is observed in numerous melanoma cells, demonstrating expression of SRY-Box Transcription Factor 10. The surrounding stromal regions show comparatively little nuclear immunoreactivity, highlighting the predominantly tumor-associated staining pattern.

SDS-PAGE analysis of purified, BSA-free SRY-box 10/SOX10 antibody (clone rSOX10/1074) as confirmation of integrity and purity.

SOX10 Antibody rSOX10/1074 by WB in Human COLO-38 Cell Lysate. Western blot analysis of human COLO-38 melanoma cell lysate using recombinant mouse monoclonal SOX10 antibody rSOX10/1074. Immunoreactive bands are detected in the approximately 40-50 kDa region, near the calculated molecular weight of 50 kDa for SRY-Box Transcription Factor 10. SOX10 has been reported to exhibit multiple electrophoretic forms, with phosphorylation contributing to differences in protein migration.

SOX10 Antibody rSOX10/1074 by Protein Microarray Specificity Analysis. Protein microarray analysis of recombinant mouse monoclonal SOX10 antibody rSOX10/1074 using a HuProt array containing more than 19,000 full-length human proteins. Fluorescence signals were ranked to evaluate antibody binding across the protein array. The specificity profile shows a prominent leading signal relative to the remaining ranked proteins, supporting preferential recognition of SRY-Box Transcription Factor 10. Z-scores represent fluorescence signal strength relative to the array-wide mean, while S-scores measure separation between ranked protein signals.