SMMHC Antibody / Smooth Muscle Differentiation Marker Antibody [clone MYH11/2303R] (V3733)

SMMHC Antibody Breast Carcinoma IHC. Immunohistochemical staining of FFPE human breast carcinoma demonstrates expression of Smooth Muscle Myosin Heavy Chain using recombinant SMMHC antibody clone MYH11/2303R. SMMHC, encoded by the MYH11 gene, is a highly specific marker of differentiated smooth muscle and myoepithelial cells. Strong cytoplasmic staining highlights residual myoepithelial cells surrounding glandular structures, consistent with the utility of SMMHC in distinguishing invasive from in situ breast lesions. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 minutes followed by cooling at room temperature for 20 minutes. These results support the utility of SMMHC Antibody for studies of myoepithelial differentiation, breast pathology, and smooth muscle biology.

SMMHC Antibody Breast Myoepithelium IHC. Immunohistochemical staining of FFPE human breast carcinoma demonstrates Smooth Muscle Myosin Heavy Chain expression using SMMHC antibody clone MYH11/2303R. SMMHC, encoded by MYH11, is a highly specific marker of mature smooth muscle and myoepithelial differentiation. Strong cytoplasmic staining is observed in myoepithelial cells surrounding ductal structures within adipose-rich breast tissue, while adjacent stromal elements remain largely negative. This staining pattern highlights the value of SMMHC in identifying intact myoepithelial layers and aiding in the distinction between invasive and noninvasive breast lesions. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 minutes followed by cooling at room temperature for 20 minutes. These results support the utility of SMMHC Antibody for studies of breast pathology, myoepithelial biology, and smooth muscle differentiation.

SMMHC Antibody Prostate Carcinoma IHC. Immunohistochemical staining of FFPE human prostate carcinoma demonstrates Smooth Muscle Myosin Heavy Chain expression using SMMHC antibody clone MYH11/2303R. SMMHC, encoded by the MYH11 gene, is a highly specific marker of differentiated smooth muscle and prostatic basal-associated stromal elements. Distinct cytoplasmic staining highlights smooth muscle bundles and vascular walls surrounding malignant glands, while neoplastic epithelial cells show little to no reactivity. Preservation or loss of SMMHC-positive structures provides valuable information in the histopathologic evaluation of prostatic lesions. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 minutes followed by cooling at room temperature for 20 minutes. These results support the utility of SMMHC Antibody for studies of prostate pathology, smooth muscle differentiation, and stromal architecture.

SDS-PAGE analysis of purified, BSA-free recombinant SMMHC antibody (clone MYH11/2303R) as confirmation of integrity and purity.

SMMHC Antibody Gastrointestinal Tissues WB. Western blot analysis of multiple gastrointestinal tissue lysates using SMMHC Antibody clone MYH11/2303R. Samples include human, mouse, rat, hamster, guinea pig, and rabbit intestine, as well as stomach tissues from the same species. MYH11, also known as Smooth Muscle Myosin Heavy Chain (SMMHC), is a contractile protein highly enriched in visceral smooth muscle cells and serves as a marker of smooth muscle differentiation. A prominent band is detected at approximately 200 kDa in human, guinea pig, and rabbit intestine, consistent with the expected molecular weight of MYH11. Species-dependent differences in signal intensity likely reflect variation in expression levels and epitope conservation. These results demonstrate the utility of MYH11 Antibody for studies of smooth muscle biology, gastrointestinal physiology, and cross-species tissue analysis.

SMMHC Antibody Stomach and Intestine Tissues WB. Western blot analysis of human intestine and human stomach tissue lysates using SMMHC Antibody clone MYH11/2303R. SMMHC, also known as Smooth Muscle Myosin Heavy Chain or MYH11, is a major contractile protein that serves as a highly specific marker of differentiated smooth muscle cells. A strong band is detected at approximately 200 kDa in both tissue samples, corresponding to the expected molecular weight of full length MYH11. The robust expression observed in gastrointestinal tissues is consistent with the abundance of smooth muscle layers responsible for peristalsis and organ motility. These results support the utility of SMMHC Antibody for studies of smooth muscle differentiation, gastrointestinal physiology, and visceral smooth muscle pathology.