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Home >> Antibodies >> Recombinant S100A4 Antibody / FSP1

Recombinant S100A4 Antibody / FSP1 [clone S100A4/2750R] (V7344)

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  Catalog No Formulation Size Price (USD)  
Image V7344-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7344-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7344SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V7344IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
Microvalidated Recrabbitmono
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Western blot testing of human HeLa cell lysate with recombinant S100A4 antibody (clone S100A4/1482). Predicted molecular weight ~12 kDa.
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Western blot testing of human HeLa cell lysate with recombinant S100A4 antibody. Predicted molecular weight ~12 kDa.
IHC testing of FFPE human placenta with recombinant S100A4 antibody. Required HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Immunofluorescent staining of permeabilized human A549 cells with recombinant S100A4 antibody (green, clone S100A4/2750R) and Reddot (red, nuclear stain).
FACS testing of permeabilized human A549 cells with recombinant S100A4 antibody (blue, clone S100A4/2750R) and isotype control (red).
SDS-PAGE analysis of purified, BSA-free recombinant S100A4 antibody as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name S100A4/2750R
Purity Protein A affinity chromatography
UniProt P26447
Localization Cytoplasmic, nuclear
Applications Western blot : 1-2ug/ml
Flow cytometry : 1-2ug/10^6 cells
Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This recombinant S100A4 antibody is available for research use only.
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Description

S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, 64 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.

Application Notes

The optimal dilution of the recombinant S100A4 antibody for each application should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A portion of amino acids 1-200 from the human protein was used as the immunogen for this recombinant S100A4 antibody.

Storage

Store the recombinant S100A4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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