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Home >> Antibodies >> Recombinant RPA32 Antibody / Replication Protein A2

Recombinant RPA32 Antibody / Replication Protein A2 [clone RPA2/3140R] (V7886)

  Catalog No Formulation Size Price (USD)  
Image V7886-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7886-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7886SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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Western blot testing of human MCF7 and T47D cell lysate with recombinant RPA32 antibody. Expected molecular weight ~32 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name RPA2/3140R
Purity Protein A affinity chromatography
UniProt P15927
Applications Western blot : 1-2ug/ml
Limitations This recombinant RPA32 antibody is available for research use only.
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Description

Recognizes a protein of 34kDa, identified as a subunit of Replication Protein A (RPA) (also known as human single-stranded DNA binding protein, or HSSB). RPA from human cells is a stable heterotrimer of 70kDa, 32-34kDa, and 11-14kDa subunits (RPA70, RPA32, and RPA14 respectively). It is involved in DNA replication, repair, and recombination. RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase (pol) alpha and delta. RPA34 is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV radiation.

Application Notes

Optimal dilution of the recombinant RPA32 antibody should be determined by the researcher.

Immunogen

A recombinant human full-length Replication protein A2 protein was used as the immunogen for this recombinant RPA32 antibody.

Storage

Store the recombinant RPA32 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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