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Home >> Antibodies >> Recombinant Rb1 Antibody / Retinoblastoma

Recombinant Rb1 Antibody / Retinoblastoma [clone rRB1/8060] (V4326)

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  Catalog No Formulation Size Price (USD)  
Image V4326-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4326-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4326SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC staining of FFPE human colon carcinoma tissue with recombinant Retinoblastoma antibody (clone rRB1/8060). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon carcinoma tissue with recombinant Retinoblastoma antibody (clone rRB1/8060). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rRB1/8060
Purity Protein A/G affinity
UniProt P06400
Localization Nucleus
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This recombinant Rb1 antibody is available for research use only.
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Description

Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. Its epitope is localized between aa 703-772. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.

Application Notes

Optimal dilution of the recombinant Rb1 antibody should be determined by the researcher.

Immunogen

A recombinant human protein fragment of 283 amino acids was used as the immunogen for the recombinant Rb1 antibody. Its epitope is localized between amino acids 703-772.

Storage

Aliquot the recombinant Rb1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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