Rabbit IgG Fc Antibody for ELISA / Anti-Rabbit IgG Fc Detection Antibody [clone RMG02] (R20173)

Rabbit IgG Fc Antibody for WB Analysis. Western blot analysis of rabbit IgG under non-reducing (-) and reducing (+) conditions demonstrates that clone RMG02 detects a band at approximately 150 kDa in the non-reduced sample, consistent with intact rabbit IgG. No significant signal is observed under reducing conditions, supporting Fc-region-dependent recognition that is sensitive to denaturation of the antibody structure. Lane 1: non-reduced rabbit IgG, Lane 2: reduced rabbit IgG. This result confirms selective recognition of native IgG and highlights the utility of this Rabbit IgG Fc Antibody / Anti-Rabbit IgG Fc Detection Antibody in applications requiring Fc-specific detection.

Rabbit IgG Fc Antibody for ELISA Species Specificity Analysis. ELISA analysis of IgG from multiple species demonstrates that clone RMG02 selectively recognizes rabbit IgG and rabbit IgG Fc, with strong signal observed across tested concentrations, while no detectable binding is observed with human, mouse, or rat IgG. This result confirms Fc-region-specific recognition and strict species selectivity. The Rabbit IgG Fc Antibody for ELISA / Anti-Rabbit IgG Fc Detection Antibody enables accurate detection of rabbit IgG in multi-species samples and supports high-specificity ELISA workflows with minimal background.

Rabbit IgG Fc Antibody for ELISA Titration Curve. ELISA titration analysis using plates coated with serial dilutions of rabbit IgG demonstrates strong, concentration-dependent binding of clone RMG02 across a broad dynamic range. Signal intensity decreases proportionally with both antigen concentration and antibody dilution, confirming sensitive and reproducible Fc-region-specific recognition of rabbit IgG. Detection was performed using an alkaline phosphatase-conjugated anti-goat IgG secondary antibody. This result supports reliable quantification and consistent performance of the Rabbit IgG Fc Antibody for ELISA / Anti-Rabbit IgG Fc Detection Antibody in ELISA-based assays.