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Home >> Antibodies >> Recombinant PSA Antibody / Prostate Specific Antigen

Recombinant PSA Antibody / Prostate Specific Antigen [clone KLK3/4602R] (V9540)

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  Catalog No Formulation Size Price (USD)  
Image V9540-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 539
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V9540-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 249
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V9540SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
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IHC staining of FFPE human prostate carcinoma tissue with recombinant PSA antibody (clone KLK3/4602R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human prostate carcinoma tissue with recombinant PSA antibody (clone KLK3/4602R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate carcinoma tissue with recombinant PSA antibody (clone KLK3/4602R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC staining of FFPE human brain tissue with recombinant PSA antibody (clone KLK3/4602R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name KLK3/4602R
Purity Protein A/G affinity
UniProt P07288
Localization Secreted
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant PSA antibody is available for research use only.
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Description

Prostate-specific antigen (PSA) is a single-chain glycoprotein of 237 amino acids containing approximately 8% carbohydrate. It is a serine protease produced almost exclusively by prostatic epithelial cells. Immunohistochemically PSA is expressed in the highly specialized apically-superficial layer of female and male secretory cells of the prostate gland, and is readily demonstrated in adenocarcinomas of the prostate in about 99% of the cases. There is a correlation between malignancy grade and intensity of staining, high grade carcinomas displaying weaker expression. About 1% of poorly differentiated carcinomas have been negative for PSA. Due to the high specificity of PSA for prostatic glandular epithelium, it is very useful in identifying prostatic carcinoma in the prostate and in the adjacent organs often affected by epithelial malignancies, i.e. rectum and urinary bladder. PSA may be used in a panel together with NKX3.1 and Prostein, which are at least as sensitive and slightly more specific than PSA.

Application Notes

Optimal dilution of the recombinant PSA antibody should be determined by the researcher.

Immunogen

A portion of amino acids 150-250 of Prostate-specific antigen protein was used as the immunogen for the recombinant PSA antibody.

Storage

Aliquot the recombinant PSA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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