Prostein Antibody / Prostate Epithelial Differentiation Marker Antibody [clone ZR9] (V8600)

Prostein Antibody for IHC. Immunohistochemistry analysis of Prostein / SLC45A3 antibody staining in FFPE human prostate carcinoma using clone ZR9. Strong cytoplasmic and perinuclear staining is observed in tumor epithelial cells forming glandular structures, consistent with Golgi-associated localization of Prostein, while surrounding stromal components remain largely negative. The staining highlights prostate epithelial differentiation within malignant glands and preserves luminal cell architecture, supporting the role of SLC45A3 as a prostate lineage marker in carcinoma tissue. Heat-induced epitope retrieval was performed in pH 9 Tris-EDTA buffer for 20 minutes followed by cooling prior to antibody incubation.

IHC staining of FFPE human prostate with recombinant Prostein antibody (clone ZR9). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Prostein Antibody Microarray Specificity Validation. Analysis of HuProt(TM) protein microarray using Prostein / SLC45A3 antibody (clone ZR9). Screening against more than 19,000 full-length human proteins demonstrates strong and selective binding to SLC45A3 as the top-ranked target, with minimal off-target reactivity across the array, supporting high specificity of the ZR9 monoclonal antibody. Signal strength is represented by the Z-score, which reflects standard deviations above the mean signal intensity across all proteins, while the S-score represents the difference in signal between the top-ranked target and the next highest signal, indicating relative binding specificity.