Prostein Antibody / Microarray-Validated Specificity Antibody [clone SLC45A3/7176R] (V9764)

Prostein Antibody Microarray Specificity Validation. Analysis of HuProt(TM) protein microarray using Prostein / SLC45A3 antibody (clone SLC45A3/7176R). Screening against more than 19,000 full-length human proteins identifies SLC45A3 as the top-ranked target with the highest signal intensity, demonstrating strong and selective binding with minimal off-target interactions across the array. Signal strength is represented by the Z-score, which reflects standard deviations above the mean signal intensity for all proteins on the array, while the S-score represents the difference between the top-ranked signal and the next highest signal, indicating relative binding specificity and target selectivity of the antibody.

Prostein Antibody for IHC. Immunohistochemistry analysis of Prostein / SLC45A3 antibody staining in FFPE human prostate tissue using clone SLC45A3/7176R. Strong cytoplasmic and perinuclear staining is observed in prostate glandular epithelial cells lining luminal structures, consistent with Golgi-associated localization of Prostein, while surrounding stromal cells remain negative. The negative control inset using PBS in place of primary antibody shows no staining, confirming specificity of the signal. This staining pattern supports selective detection of SLC45A3 in prostate epithelial cells in immunohistochemistry applications. Heat-induced epitope retrieval was performed in pH 9 Tris-EDTA buffer for 20 minutes followed by cooling prior to antibody incubation.

SDS-PAGE analysis of purified, BSA-free recombinant Prostein antibody (clone SLC45A3/7176R) as confirmation of integrity and purity.