PMS2 Antibody / DNA Mismatch Repair Protein Antibody [clone PMS2/4373R] (V8728)

PMS2 Antibody Human Colon Carcinoma IHC. Immunohistochemical staining of FFPE human colon carcinoma tissue using recombinant PMS2 antibody demonstrated strong nuclear staining in tumor cells, consistent with the role of PMS2 as a DNA mismatch repair protein. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 Tris-EDTA buffer (10 mM Tris, 1 mM EDTA) for 20 minutes followed by cooling prior to staining. The predominantly nuclear staining pattern is consistent with the localization of PMS2 within the DNA mismatch repair machinery. This PMS2 Antibody, also called a DNA Mismatch Repair Protein Antibody, is useful for investigating mismatch repair, genomic stability, Lynch syndrome, and microsatellite instability.

PMS2 Antibody Human Colorectal Cancer IHC. Immunohistochemical staining of FFPE human colon carcinoma tissue using recombinant PMS2 antibody demonstrated strong nuclear staining in malignant epithelial cells, consistent with the nuclear localization of PMS2 as a DNA mismatch repair protein. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 Tris-EDTA buffer (10 mM Tris, 1 mM EDTA) for 20 minutes followed by cooling prior to staining. The preserved nuclear immunoreactivity is consistent with intact PMS2 expression in this tumor. This PMS2 Antibody, also called a DNA Mismatch Repair Protein Antibody, is useful for investigating mismatch repair, genomic stability, Lynch syndrome, and microsatellite instability.

PMS2 Antibody Protein Microarray Validation. Human HuProt protein microarray analysis containing more than 19,000 full-length human proteins demonstrated exceptional specificity of recombinant PMS2 antibody (clone PMS2/4373R). PMS2 was identified as the highest-ranking target with a Z-score of 134.71 and an S-score of 75.9, substantially exceeding all other proteins on the array. The Z-score reflects the strength of antibody binding to an individual protein relative to background, while the S-score measures the specificity of the antibody for its intended target compared with the next highest-ranking proteins. These results confirm the excellent target specificity of clone PMS2/4373R for research applications.