- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
PLAP Antibody / ALPP N-Terminal Epitope Antibody [clone RM317] (R20336)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
R20336-0.1ML | Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide | 100 ul | 439 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG |
| Clone Name | RM317 |
| Purity | Protein A purified from animal origin-free supernatant |
| UniProt | P05187 |
| Localization | Cytoplasmic, cell membrane |
| Applications | Immunohistochemistry (FFPE) : 1:500-1:1000 Western Blot : 1:1000-1:2000 |
| Limitations | This PLAP Antibody / ALPP N-Terminal Epitope Antibody is available for research use only. |
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Placental alkaline phosphatase (ALPP), commonly known as PLAP, is a glycosylphosphatidylinositol-anchored membrane glycoprotein belonging to the alkaline phosphatase enzyme family. The protein is strongly associated with placental trophoblasts, epithelial differentiation pathways, and germ cell tumor biology, where it functions in extracellular phosphomonoester hydrolysis and membrane-associated phosphate metabolism. PLAP Antibody is useful for studies involving placental epithelial biology, trophoblast-associated differentiation, and tumor lineage characterization in epithelial and germ cell-derived tissues.
PLAP antibody, also referred to as Placental alkaline phosphatase antibody and ALPP antibody in the literature, recognizes a tissue-restricted alkaline phosphatase isozyme encoded on chromosome 2q37.1. ALPP localizes predominantly to the plasma membrane and extracellular cellular surface through a glycosylphosphatidylinositol anchor. Because placental alkaline phosphatase undergoes substantial glycosylation and extracellular membrane processing, epitope localization may influence antibody accessibility, staining characteristics, and recognition of processed protein forms in tissue and cell-based assays.
PLAP Antibody / ALPP N-Terminal Epitope Antibody (clone RM317) is useful for studies involving placental membrane biology, trophoblast differentiation pathways, epithelial membrane organization, and germ cell tumor-associated signaling mechanisms. This recombinant rabbit monoclonal PLAP Antibody is designed to recognize an N-terminal epitope of placental alkaline phosphatase / ALPP, providing an alternative detection profile relative to antibodies directed against other extracellular regions of the protein. N-terminal epitope targeting may support recognition of membrane-associated and processed PLAP species in epithelial and trophoblast-associated cellular populations.
Placental alkaline phosphatase expression is strongly associated with syncytiotrophoblasts and trophoblast-derived epithelial compartments, where the protein contributes to extracellular phosphate metabolism and membrane-associated phosphatase activity. In pathology-focused research, PLAP is widely recognized as a marker associated with seminoma and germ cell tumor differentiation. The restricted normal tissue distribution of ALPP relative to other alkaline phosphatase family members contributes to its value in studies examining tumor lineage identity and trophoblastic differentiation programs.
ALPP is heavily glycosylated and commonly appears at higher apparent molecular weights than predicted from amino acid sequence alone. Membrane localization, extracellular domain structure, and post-translational processing may influence epitope accessibility in western blot, immunofluorescence, flow cytometry, and tissue-based applications. N-terminal epitope-directed antibodies may therefore provide distinct staining characteristics depending on fixation conditions, protein processing state, and membrane orientation within target cells and tissues.
PLAP Antibody supports research involving placental biology, trophoblast differentiation pathways, germ cell tumor markers, epithelial membrane proteins, extracellular phosphate metabolism, and alkaline phosphatase-associated signaling mechanisms. The antibody may be incorporated into investigations examining placental development, reproductive tissue pathology, epithelial differentiation state, and membrane-associated protein regulation in normal and diseased tissues.
For a clone-defined placental marker antibody with extensive placenta-positive and normal tissue-negative immunohistochemistry validation data, see our ALPP Antibody / Placental Marker Antibody page featuring clone rALP/870.
The stated application concentrations are suggested starting points. Titration of the PLAP Antibody / ALPP N-Terminal Epitope Antibody may be required due to differences in protocols and secondary/substrate sensitivity.
A peptide corresponding to the N-terminus of human placental alkaline phosphatase was used as the immunogen for the recombinant PLAP antibody.
Store the PLAP antibody at -20oC.
PLAP antibody, ALPP antibody, Placental alkaline phosphatase antibody, N-terminal PLAP antibody, ALPP N-terminus antibody
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