Phosphotyrosine Antibody / P-Tyr [clone RM111] (R20195)

Description

Phosphotyrosine Antibody recognizes tyrosine-phosphorylated residues on proteins, a critical post-translational modification involved in cellular signaling, growth control, differentiation, and immune regulation. Protein tyrosine phosphorylation is dynamically regulated by protein tyrosine kinases and phosphatases and serves as a central mechanism for transmitting extracellular signals to intracellular pathways. Phosphotyrosine Antibody is widely used in research to monitor global tyrosine phosphorylation levels and to identify activated signaling proteins in both normal and disease-related contexts.

Tyrosine phosphorylation plays a pivotal role in receptor-mediated signaling, including pathways initiated by receptor tyrosine kinases such as EGFR, insulin receptor, and PDGF receptor. Upon ligand binding, these receptors undergo autophosphorylation on tyrosine residues, creating docking sites for downstream signaling proteins. Phosphotyrosine Antibody enables detection of these phosphorylation events, supporting studies of signal transduction, kinase activity, and pathway activation across diverse experimental systems.

In cancer research, aberrant tyrosine phosphorylation is a hallmark of oncogenic signaling, often resulting from kinase overactivation, mutation, or dysregulated phosphatase activity. Phosphotyrosine Antibody is therefore frequently applied in investigations of tumor signaling networks, drug response mechanisms, and kinase inhibitor efficacy. Its ability to broadly detect phosphotyrosine-modified proteins makes it a valuable tool for profiling signaling alterations associated with malignant transformation.

Beyond oncology, phosphotyrosine signaling is essential in immune cell activation, neuronal signaling, and developmental biology. Phosphotyrosine Antibody supports studies examining immune receptor activation, synaptic signaling events, and developmental pathway regulation by enabling visualization and analysis of tyrosine phosphorylation patterns in cells and tissues under various experimental conditions.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Phosphotyrosine antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Immunogen

Phosphotyrosine-BSA conjugate was used as the immunogen for this recombinant Phosphotyrosine antibody.

Storage

Store the recombinant Phosphotyrosine antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).