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Home >> Antibodies >> Recombinant Phosphothreonine Antibody

Recombinant Phosphothreonine Antibody [clone RM102] (R20193)

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  Catalog No Formulation Size Price (USD)  
Image R20193-100UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ug 469
Microvalidated Recrabbitmono
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ICC of serum-starved human A431 cells non-treated or treated with Calyculin A/Okadaic Acid, using the recombinant Phosphothreonine antibody at 1:500 (PE-secondary, red) and DAPI (blue).
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ICC of serum-starved human A431 cells non-treated or treated with Calyculin A/Okadaic Acid, using the recombinant Phosphothreonine antibody at 1:500 (PE-secondary, red) and DAPI (blue).
Western blot of serum-starved human A431 cells non-treated or treated with Calyculin A/Okadaic Acid, using the recombinant Phosphothreonine antibody at 1:2000.
IP of Calyculin A/Okadaic Acid treated human A431 cells by the recombinant Phosphothreonine antibody at 1:500, and then blotted with same mAb at 1:2000. (1) Whole lysate control; (2) IP by rabbit IgG control; (3) IP by RM102.
The recombinant Phosphothreonine antibody recognizes phosphorylated threonine in peptides with different sequences. It has minimal cross-reactivity with phosphorylated serine.
Availability 1-3 business days
Species Reactivity All Species
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM102
Purity Protein A purified from animal origin-free supernatant
Gene ID N/A
Applications Western blot : 1:500 -1:2000 dilution
Immunoprecipitation : 1:100-1:500 dilution
Chromatin IP (ChIP) : 1:100-1:500 dilution
Immunocytochemistry : 1:100-1:500 dilution
Immunohistochemistry : 1:100-1:500 dilution (1)
Limitations This recombinant Phosphothreonine antibody is available for research use only.
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Description

This recombinant Phosphothreonine antibody reacts with threonine-phosphorylated proteins. No cross reactivity with nonphosphorylated threonine, phosphoserine, and phosphotyrosine. It shows slight cross-reactivity with a few phospho-serine-containing peptides.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Phosphothreonine antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Immunogen

Mixture of phosphothreonine-BSA conjugate and a phosphothreonine containing peptide was used as the immunogen for this recombinant Phosphothreonine antibody.

Storage

Store the recombinant Phosphothreonine antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Related Products

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   2.   View all Phosphothreonine antibodies

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