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Home >> Antibodies >> Recombinant phospho-p38 Antibody (Thr180/Tyr182)

Recombinant phospho-p38 Antibody (Thr180/Tyr182) [clone RM243] (R20264)

  Catalog No Formulation Size Price (USD)  
Image R20264-0.1ML Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ul 419
Microvalidated Recrabbitmono
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Western blot test of HeLa cell lysate, treated or untreated with anisomycin, using recombinant phospho-p38 antibody at 1:2000.
IHC testing of FFPE human breast cancer tissue with recombinant phospho-p38 antibody at 1:1000.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM243
Purity Protein A purified from animal origin-free supernatant
UniProt Q16539
Gene ID 1432
Localization Nuclear, cytoplasmic
Applications Immunohistochemistry (FFPE) : 1:500-1:1000 (1)
Western blot : 1:1000-1:2000
Limitations This recombinant phospho-p38 antibody is available for research use only.
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Description

This recombinant phospho-p38 antibody reacts to p38 MAPK only when dual phosphorylation at Thr180 and Tyr182 is observed. There is no cross-reactivity with p38 MAPK without dual phosphorylation at Thr180 and Tyr182.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant phospho-p38 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Immunogen

A phospho-peptide corresponding to human phospho-p38 MAPK (Thr180/Tyr182) was used as the immunogen for this recombinant phospho-p38 antibody.

Storage

Store the recombinant phospho-p38 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

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