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Home >> Antibodies >> Recombinant phospho-Histone H4 Antibody (Ser1)

Recombinant phospho-Histone H4 Antibody (Ser1) [clone RM194] (R20222)

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  Catalog No Formulation Size Price (USD)  
Image R20222-100UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ug 469
Image
R20222-25UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 25 ug 239
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Western blot of (1) acid extracts of HeLa cells and (2) recombinant Histone H4 using the recombinant phospho-Histone H4 antibody at 0.5 ug/ml showed a band of Histone H4 phosphorylated at Serine 1 in HeLa cells.
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Western blot of (1) acid extracts of HeLa cells and (2) recombinant Histone H4 using the recombinant phospho-Histone H4 antibody at 0.5 ug/ml showed a band of Histone H4 phosphorylated at Serine 1 in HeLa cells.
The recombinant phospho-Histone H4 antibody specifically reacts to Histone H4 phosphorylated at Ser1 (H4S1p). The reactivity is not affected by neighboring Arg3 modifications (H4S1pR3me1 and H4S1pR3me2). No cross reactivity with Histone H2A phosphorylated at Ser1 (H2AS1p).
ICC/IF of HeLa cells using recombinant phospho-Histone H4 antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Availability 1-3 business days
Species Reactivity All Species
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM194
Purity Protein A purified from animal origin-free supernatant
UniProt P62805
Gene ID 121504
Applications Western blot : 0.5-2ug/ml
Immunocytochemistry : 1-2ug/ml
ELISA : 0.2-1ug/ml
Limitations This recombinant phospho-Histone H4 antibody is available for research use only.
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Description

This recombinant phospho-Histone H4 antibody reacts to Histone H4 phosphorylated at Serine 1. The reactivity is not affected by neighbor Arginine 3 modification. No cross reactivity with Histone H2A phosphorylated at Serine 1.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant phospho-Histone H4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A phospho-peptide corresponding to phospho-Histone H4 (pSer1) was used as the immunogen for this recombinant phospho-Histone H4 antibody.

Storage

Store the recombinant phospho-Histone H4 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

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