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Home >> Antibodies >> Recombinant phospho-Histone H2A/H4 Antibody (Ser1)

Recombinant phospho-Histone H2A/H4 Antibody (Ser1) [clone RM216] (R20231)

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  Catalog No Formulation Size Price (USD)  
Image R20231-100UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ug 469
Image
R20231-25UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 25 ug 239
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Western blot test of acid extracts of HeLa cells treated (1) or non-treated (2) with Nocodazole, using recombinant phospho-Histone H2A/H4 antibody at 0.5 ug/ml, showed both Histone H2A and H4 phosphorylated at Serine 1 in HeLa cells.
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Western blot test of acid extracts of HeLa cells treated (1) or non-treated (2) with Nocodazole, using recombinant phospho-Histone H2A/H4 antibody at 0.5 ug/ml, showed both Histone H2A and H4 phosphorylated at Serine 1 in HeLa cells.
This recombinant phospho-Histone H2A/H4 antibody specifically reacts to both Histone H2A and H4 phosphorylated at Serine 1 (H2AS1p and H4S1p).
ICC/IF staining of HeLa cells using recombinant phospho-Histone H2A/H4 antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Availability 1-3 business days
Species Reactivity All Species
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM216
Purity Protein A purified from animal origin-free supernatant
UniProt P16104 , P62805
Gene ID 3012 , 121504
Applications Western blot : 0.5-2ug/ml
Immunocytochemistry : 1-2ug/ml
ELISA : 0.2-1ug/ml
Limitations This recombinant phospho-Histone H2A/H4 antibody is available for research use only.
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Description

This recombinant phospho-Histone H2A/H4 antibody reacts to Histone H2A or Histone H4 phosphorylated at Serine 1. No cross reactivity with other phosphorylated Histones.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant phospho-Histone H2A/H4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A phospho-peptide corresponding to phospho-Histone H2A (pSer1) was used as the immunogen for this recombinant phospho-Histone H2A/H4 antibody.

Storage

Store the recombinant phospho-Histone H2A/H4 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Related Products

   1.   View all Recombinant antibodies

   2.   View all phospho-Histone H2A/H4 antibodies

   3.   View all Histone H2A antibodies

   4.   View all Histone H4 antibodies

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