- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
PHH3 Antibody / Histone H3 (pSer10) [clone PHH3/471R] (V8647)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
V8647-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 559 | |
|
|
V8647-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 259 | |
|
|
V8647SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
|
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Mouse |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Mouse IgG2a, kappa |
| Clone Name | PHH3/471R |
| Purity | Protein G affinity chromatography |
| UniProt | P68431 |
| Localization | Nucleus |
| Applications | Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT |
| Limitations | This PHH3 antibody is available for research use only. |
|
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PHH3 Antibody detects Phosphohistone H3 (PHH3), a marker specific for cells undergoing mitosis. Serine 10 of Histone H3 is phosphorylated in association with mitotic chromatin condensation in late G2 and M phase of the cell cycle and thus, PHH3 can distinguish mitosis from apoptotic nuclei. The range of percentage PHH3 positive tumor nuclei was from 0.0 to 6.6% (median value 0.8%). Increased expression of PHH3 was significantly associated with tumor thickness (p = 0.031), presence of tumor ulceration (p =0.041) and tumor necrosis (p =0.027), but not with Clarks level of invasion. High levels of PHH3 was associated with increased mitotic count (p = 0.003) and high Ki-67 expression (p = 0.002). For central nervous system tumors, melanoma, soft tissue tumors, GIST, etc., PHH3 mAb is helpful for tumor pathological classification and prognosis. This antibody is part of a broader collection of Histone H3 antibodies used to study chromatin structure, histone modifications, and epigenetic regulation.
Optimal dilution of the recombinant PHH3 antibody should be determined by the researcher.
A synthetic peptide corresponding to the amino acids surrounding phospho S10 were used as the immunogen for the recombinant PHH3 antibody.
Store the recombinant PHH3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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