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Home >> Antibodies >> Recombinant PGP9.5 Antibody

Recombinant PGP9.5 Antibody [clone UCHL1/4556R] (V8653)

  Catalog No Formulation Size Price (USD)  
Image V8653-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V8653-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V8653SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human cerebellum with recombinant PGP9.5 antibody (clone UCHL1/4556R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human cerebellum with recombinant PGP9.5 antibody (clone UCHL1/4556R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human brain lysate with recombinant PGP9.5 antibody. Predicted molecular weight ~25 kDa.
SDS-PAGE analysis of purified, BSA-free recombinant PGP9.5 antibody (clone UCHL1/4556R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name UCHL1/4556R
Purity Protein A affinity chromatography
UniProt P09936
Localization Cytoplasmic, ER membrane
Applications Western blot : 2-4ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This recombinant PGP9.5 antibody is available for research use only.
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Description

This MAb reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinsons disease.

Application Notes

Optimal dilution of the recombinant PGP9.5 antibody should be determined by the researcher.

Immunogen

Recombinant full-length human protein was used as the immunogen for the recombinant PGP9.5 antibody.

Storage

Store the recombinant PGP9.5 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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