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Home >> Antibodies >> Recombinant Perilipin 2 Antibody / PLIN2 / Adipophilin / ADRP

Recombinant Perilipin 2 Antibody / PLIN2 / Adipophilin / ADRP [clone ADPN1-2R] (V3974)

  Catalog No Formulation Size Price (USD)  
Image V3974-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 459
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V3974-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 209
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V3974SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 459
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V3974IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 459
Western blot testing of human liver lysate with recombinant Perilipin 2 antibody (clone ADPN1-2R). Predicted molecular weight ~48 kDa.
IHC testing of FFPE human adrenal tissue with recombinant Perilipin 2 antibody (clone ADPN1-2R). Required HIER: steam sections in pH6, 10mM citrate buffer for 10-20 min.
Availability 1-3 business days
Species Reactivity Human. Others not tested.
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name ADPN1-2R
Purity Protein A affinity chromatography
UniProt Q99541
Localization Cytoplasmic
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Prediluted IHC only format : incubate for 30 min at RT (1)
Western blot : 1-2ug/ml
Limitations This recombinant Perilipin 2 antibody is available for research use only.
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Description

The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene. [RefSeq]

Application Notes

Titering of the recombinant Perilipin 2 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A portion of amin acids 249-376 from the human protein were used as the immunogen for this recombinant Perilipin 2 antibody.

Storage

The recombinant Perilipin 2 antibody (with azide) can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

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