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Home >> Antibodies >> Recombinant PDPN Antibody / Podoplanin / Gp36

Recombinant PDPN Antibody / Podoplanin / Gp36 [clone rPDPN/6994] (V4681)

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  Catalog No Formulation Size Price (USD)  
Image V4681-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4681-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4681SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC staining of FFPE human tonsil tissue with recombinant PDPN antibody (clone rPDPN/6994). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human tonsil tissue with recombinant PDPN antibody (clone rPDPN/6994). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant PDPN antibody (clone rPDPN/6994) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rPDPN/6994
Purity Protein A/G affinity
UniProt Q86YL7
Localization Cell Surface, Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This recombinant PDPN antibody is available for research use only.
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Description

It recognizes a muco-protein of 38-43kDa, which is identified as Podoplanin (PDPN). It localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. It is selectively expressed in lymphatic endothelium as well as lymphoangiomas, Kaposi sarcomas, and in a subset of angiosarcomas with probable lymphatic differentiation. Recent studies have also shown podoplanin to be a highly sensitive and relatively specific marker for epithelioid mesothelioma. Therefore, it can be used in a panel to distinguish mesotheliomas or mesothelial cells from pulmonary carcinomas.

Application Notes

Optimal dilution of the recombinant PDPN antibody should be determined by the researcher.

Immunogen

A recombinant human Podoplanin protein fragment (within amino acids 1-100) was used as the immunogen for the recombinant PDPN antibody.

Storage

Aliquot the recombinant PDPN antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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