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Home >> Antibodies >> Recombinant PCNA Antibody / Proliferating Cell Nuclear Antigen

Recombinant PCNA Antibody / Proliferating Cell Nuclear Antigen [clone rPCNA/8858] (V4825)

  Catalog No Formulation Size Price (USD)  
Image V4825-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4825-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4825SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC staining of FFPE human tonsil tissue with recombinant PCNA antibody (clone rPCNA/8858) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with recombinant PCNA antibody (clone rPCNA/8858) at 2ug/ml. Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rPCNA/8858
Purity Protein A/G affinity
UniProt P12004
Localization Nucleus, Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This recombinant PCNA antibody is available for research use only.
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Description

Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This mAb is excellent for multiple applications.

Application Notes

Optimal dilution of the recombinant PCNA antibody should be determined by the researcher.

Immunogen

Recombinant full-length human PCNA protein was used as the immunogen for the recombinant PCNA antibody.

Storage

Aliquot the recombinant PCNA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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