PAX8 Antibody / Thyroid Differentiation Marker Antibody [clone PAX8/2774R] (V7465)

PAX8 Antibody / Thyroid Differentiation Marker Antibody immunohistochemistry in human thyroid tissue showing strong nuclear HRP-DAB brown staining in follicular epithelial cells. Paired box protein Pax-8 (PAX8) expression is localized to nuclei of thyroid follicular cells lining colloid-filled follicles, with clear sparing of colloid material and surrounding stromal tissue. The staining pattern highlights dense follicular epithelial populations with expected nuclear positivity, while non-epithelial cells remain negative with hematoxylin-only nuclear counterstain. This distinct nuclear localization supports the role of PAX8 as a thyroid lineage transcription factor and reinforces its utility as a differentiation marker in thyroid tissue analysis. Heat-induced epitope retrieval was performed by boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 minutes followed by cooling prior to antibody incubation.

PAX8 Antibody / Thyroid Differentiation Marker Antibody immunohistochemistry in human kidney tissue showing nuclear HRP-DAB brown staining in renal tubular epithelial cells. Paired box protein Pax-8 (PAX8) expression is localized to nuclei of tubular epithelial cells, with clear staining of nephron structures and minimal signal in surrounding stromal components. The presence of nuclear positivity in kidney tissue reflects shared epithelial lineage expression of PAX8 and highlights the importance of tissue context when interpreting thyroid differentiation markers. Background cells remain largely negative with hematoxylin-only nuclear staining. Required HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant PAX8 antibody as confirmation of integrity and purity.