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Home >> Antibodies >> Recombinant PAX8 Antibody

Recombinant PAX8 Antibody [clone PAX8/2774R] (V7465)

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  Catalog No Formulation Size Price (USD)  
Image V7465-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 559
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V7465-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 259
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V7465SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
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V7465IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 559
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Immunohistochemistry analysis of PAX8 antibody in human thyroid tissue. Formalin-fixed, paraffin-embedded human thyroid sections demonstrate strong HRP-DAB brown nuclear staining in thyroid follicular epithelial cells, consistent with nuclear localization of PAX8 as a transcription factor. The staining is confined to nuclei with clear sparing of colloid material and surrounding stromal tissue, and negative cells retain blue hematoxylin counterstain without brown signal. Heat-induced epitope retrieval was performed by boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 minutes followed by cooling prior to antibody incubation.
IHC testing of FFPE human kidney with PAX8 antibody. Required HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant PAX8 antibody as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name PAX8/2774R
Purity Protein A affinity chromatography
UniProt Q06710
Localization Nuclear, cytoplasmic
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This recombinant PAX8 antibody is available for research use only.
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Description

Recombinant PAX8 antibody recognizes PAX8, a nuclear transcription factor encoded by the PAX8 gene and a critical regulator of epithelial differentiation and organ development. PAX8 belongs to the paired box family of transcription factors characterized by a conserved paired DNA-binding domain that mediates sequence-specific transcriptional control. PAX8 is essential for normal development of the thyroid gland, kidney, and Mullerian system, making it a widely studied marker in epithelial lineage research.

During embryogenesis, PAX8 contributes to specification and maturation of thyroid follicular cells and renal epithelial structures. In the thyroid, PAX8 regulates genes involved in hormone synthesis and follicular differentiation, while in the kidney it supports nephric epithelial identity. In Mullerian-derived tissues, including fallopian tube and endometrium, PAX8 participates in epithelial development and maintenance. Detection of PAX8 therefore reflects lineage commitment in these specialized epithelial compartments.

PAX8 protein localizes to the nucleus, consistent with its role as a transcription factor. In adult tissues, expression is maintained in thyroid follicular epithelium, renal tubular epithelial cells, and Mullerian epithelium. Outside of these tissues, expression is limited, supporting its value as a selective epithelial lineage marker. In tumor biology research, PAX8 expression is frequently retained in renal cell carcinoma, thyroid carcinoma, and ovarian and endometrial carcinomas derived from Mullerian epithelium. Because of this distribution, PAX8 antibody is commonly used in studies examining tumor differentiation and tissue origin.

The PAX8 gene is located on chromosome 2q14 and generates multiple isoforms through alternative splicing. Altered expression and gene rearrangements involving PAX8 have been associated with thyroid and other epithelial neoplasms. Recombinant PAX8 antibody clone PAX8/2774R is a rabbit monoclonal antibody developed to detect PAX8 in research applications. As a recombinant monoclonal antibody, clone PAX8/2774R supports consistent nuclear detection of PAX8 protein in studies of developmental biology, epithelial differentiation, and tumor lineage analysis.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant PAX8 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A human recombinant fragment (aa 60-261) was used as the immunogen for the recombinant PAX8 antibody.

Storage

Store the recombinant PAX8 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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