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Home >> Antibodies >> Recombinant Pan-HLA Antibody (DP/DQ/DR)

Recombinant Pan-HLA Antibody (DP/DQ/DR) [clone rHLA-Pan/3475] (V8305)

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  Catalog No Formulation Size Price (USD)  
Image V8305-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V8305-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V8305SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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Flow cytometry testing of human Raji cells with recombinant Pan-HLA antibody (clone rHLA-Pan/3475); Red=isotype control, Blue= recombinant Pan-HLA antibody.
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Flow cytometry testing of human Raji cells with recombinant Pan-HLA antibody (clone rHLA-Pan/3475); Red=isotype control, Blue= recombinant Pan-HLA antibody.
IHC testing of FFPE human tonsil tissue with recombinant Pan-HLA antibody (clone rHLA-Pan/3475). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool prior to testing.
Immunofluorescence staining of human Raji cells with recombinant Pan-HLA antibody (green, clone rHLA-Pan/3475) and Reddot nuclear stain (red).
SDS-PAGE analysis of purified, BSA-free recombinant Pan-HLA antibody (clone rHLA-Pan/3475) as confirmation of integrity and purity.
Western blot testing of human 1) Ramos and 2) spleen tissue lysate with recombinant Pan-HLA antibody (clone rHLA-Pan/3475).
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rHLA-Pan/3475
Purity Protein G affinity chromatography
UniProt P04440 (HLA-DP) , P01908 , P01909 , P01920 (HLA-DQ) , P01903 (HLA-DR)
Localization Cell surface
Applications Flow cytometry : 1-2ug/10^6 cells in 0.1ml
Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Western blot : 1-2ug/ml
Limitations This recombinant Pan-HLA antibody is available for research use only.
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Description

Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR. Human MHC class II antigens are transmembrane glycoproteins composed of an alpha chain (36kDa) and a beta chain (27kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six alpha and ten beta chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response.

Application Notes

Optimal dilution of the recombinant Pan-HLA antibody should be determined by the researcher.

Immunogen

The non-T, non-B human acute lymphoblastic leukemia REH6 cell line was used as the immunogen for the Pan-HLA antibody.

Storage

Store the recombinant Pan-HLA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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