p75NTR Antibody NGFR/1997R / TNFRSF16 Antibody [clone NGFR/1997R] (V3584)

Description

p75NTR Antibody NGFR/1997R recognizes the p75 neurotrophin receptor (p75NTR), a cell surface glycoprotein encoded by the NGFR gene. Also known as TNFRSF16, CD271, and nerve growth factor receptor, this protein belongs to the tumor necrosis factor receptor superfamily. It regulates neuronal survival, differentiation, apoptosis, and responses to extracellular neurotrophins. Expression in neural crest-derived cells, developing neurons, and selected non-neural tissues makes p75NTR an important marker in neuroscience, developmental biology, and cancer research.

The p75 neurotrophin receptor contains four extracellular cysteine-rich domains, a transmembrane region, and an intracellular death domain. Unlike Trk receptors, p75NTR lacks intrinsic tyrosine kinase activity and relies on interactions with signaling partners. It binds nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4). Depending on receptor complexes and cellular context, these interactions can influence neuronal survival, programmed cell death, and cytoskeletal organization.

TNFRSF16 participates in signaling pathways involving NF-kappaB, JNK, and RhoA. Association with Trk receptors can modify cellular sensitivity to neurotrophins, while interactions with sortilin and related proteins may promote responses to proneurotrophins. These signaling mechanisms contribute to neurite extension, neuronal differentiation, and cellular responses to injury. Expression of CD271 can increase following peripheral nerve damage, reflecting its involvement in regeneration and Schwann cell biology.

Beyond the nervous system, p75NTR is expressed in certain mesenchymal stromal cell populations and neural crest-associated tumors. In melanoma research, CD271 has been investigated as a marker associated with cellular differentiation, invasive behavior, and tumor heterogeneity. Its expression is particularly relevant to desmoplastic and neurotropic melanoma, where detection of nerve growth factor receptor can help characterize tumor cells with neural differentiation features.

Immunohistochemical detection of p75NTR provides information about receptor distribution in normal and malignant tissues. Recombinant monoclonal antibodies preserve a defined antibody sequence and support consistent clone identity across production batches. A p75NTR Antibody NGFR/1997R from NSJ Bioreagents supports investigations of TNFRSF16 expression, neuronal signaling, melanoma biology, and neurotrophin-associated cellular responses.

For an alternative antibody targeting the same neurotrophin receptor, see our NGFR Antibody NGFR/4919, a mouse monoclonal antibody featuring human melanoma IHC and protein microarray specificity validation.

Application Notes

Optimal dilution of the p75NTR Antibody NGFR/1997R / TNFRSF16 Antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human protein was used as the immunogen for the p75NTR Antibody NGFR/1997R.

Storage

Store the p75NTR Antibody NGFR/1997R at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

NGFR antibody, CD271 antibody, NGF Receptor antibody, Nerve Growth Factor Receptor antibody, Low Affinity NGF Receptor antibody, p75 Neurotrophin Receptor antibody, TNFRSF16 antibody