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Home >> Antibodies >> Recombinant p65 Antibody

Recombinant p65 Antibody [clone RM273] (R20290)

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  Catalog No Formulation Size Price (USD)  
Image R20290-0.1ML Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ul 419
Microvalidated Recrabbitmono
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IHC testing of FFPE human breast cancer tissue with recombinant p65 antibody at 1:1250.
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IHC testing of FFPE human breast cancer tissue with recombinant p65 antibody at 1:1250.
Western blot of human MCF7 cell lysate using recombinant p65 antibody at 1:500. Expected molecular weight ~65 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM273
Purity Protein A purified from animal origin-free supernatant
UniProt Q04206
Gene ID 5970
Localization Nuclear, cytoplasmic
Applications Immunohistochemistry (FFPE) : 1:500-1:1500 (1)
Western blot : 1:500-1:1000
Limitations This recombinant p65 antibody is available for research use only.
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Description

This recombinant p65 antibody reacts to human transcription factor p65 (NFkB p65 subunit).

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant p65 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Immunogen

A peptide corresponding to the area near the C-terminus of human transcription factor p65 (NFkB p65 subunit) was used as the immunogen for this recombinant p65 antibody.

Storage

Store the recombinant p65 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

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