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Home >> Antibodies >> Recombinant p53 Antibody / Rabbit Monoclonal

Recombinant p53 Antibody / Rabbit Monoclonal [clone TP53/1799R] (V3513)

  Catalog No Formulation Size Price (USD)  
Image V3513-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3513-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3513SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V3513IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
Microvalidated Recrabbitmono
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Protein array validation of the recombinant p53 antibody: Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using recombinant p53 antibody (clone TP53/1799R).

Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

IHC testing of FFPE human colon carcinoma with recombinant p53 antibody (clone TP53/1799R). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
SDS-PAGE analysis of purified, BSA-free recombinant p53 antibody (clone TP53/1799R) as confirmation of integrity and purity.
Western blot testing of human HeLa cell lysate with recombinant p53 antibody (clone TP53/1799R).
Flow cytometry staining of human HeLa cells with recombinant p53 antibody (clone TP53/1799R); Red=isotype control, Blue= recombinant p53 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name TP53/1799R
Purity Protein A affinity chromatography
UniProt P04637
Localization Predominantly nuclear
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Flow cytometry : 1-2ug/million cells
Limitations This recombinant p53 antibody is available for research use only.
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Description

Recognizes a 53 kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.

Application Notes

The optimal dilution of the recombinant p53 antibody for each application should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human p53 protein was used as the immunogen for this recombinant p53 antibody.

Storage

Store the recombinant p53 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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