- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
p53 Antibody Clone CTA53-2R / TP53 Tumor Suppressor Antibody [clone CTA53-2R] (V3750)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
V3750-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 559 | |
|
|
V3750-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 259 | |
|
|
V3750SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 | |
|
|
V3750IHC-7ML | Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* | 7 ml | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG, kappa |
| Clone Name | CTA53-2R |
| Purity | Protein A affinity chromatography |
| UniProt | P04637 |
| Localization | Predominantly nuclear |
| Applications | Western Blot : 1-2ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT Prediluted IHC Only Format : incubate for 30 min at RT (1) |
| Limitations | This recombinant p53 antibody is available for research use only. |
|
Review this product on BioCompare and get a $20 Amazon gift card
|
Related Products
|
Tumor protein p53 (TP53) is a sequence-specific transcription factor that functions as a central tumor suppressor regulating DNA damage responses, cell cycle arrest, apoptosis, and genomic stability. The p53 Antibody Clone CTA53-2R is a recombinant rabbit monoclonal antibody developed for detection of p53 expression and investigation of TP53 signaling pathways involved in cancer biology and cellular stress responses.
TP53 antibody, also known as Tumor protein p53 antibody or Cellular tumor antigen p53 antibody in the literature, targets one of the most extensively studied tumor suppressor proteins in human biology. The TP53 gene is located on chromosome 17p13.1 and encodes a transcription factor belonging to the p53 family of DNA-binding proteins. The p53 protein contains multiple structural domains including an N-terminal transcriptional activation region, a central DNA-binding domain responsible for sequence-specific transcriptional regulation, a tetramerization domain required for formation of active p53 complexes, and a C-terminal regulatory region that modulates DNA interaction and protein stability.
Under normal cellular conditions, p53 protein levels are tightly controlled through rapid ubiquitination and proteasomal degradation mediated primarily by the E3 ubiquitin ligase MDM2. Cellular stress signals such as DNA damage, oncogene activation, oxidative stress, or hypoxia disrupt this regulatory pathway and stabilize p53 protein. Stabilized p53 accumulates within the nucleus where it activates transcription of genes including CDKN1A (p21), BAX, and PUMA that regulate cell cycle arrest and apoptosis. Antibodies such as p53 Antibody Clone CTA53-2R enable investigators to monitor these stress-induced changes in p53 abundance and nuclear localization.
The p53 Antibody Clone CTA53-2R recognizes Tumor protein p53 and supports detection of endogenous TP53 protein in studies examining tumor suppressor signaling and p53 pathway activation. Detection of p53 accumulation is particularly relevant in cancer biology, as many TP53 mutations lead to stabilization and nuclear retention of the p53 protein within tumor cells.
Clone CTA53-2R provides researchers with a recombinant monoclonal reagent for studying TP53 expression across diverse experimental systems. The p53 Antibody Clone CTA53-2R enables investigation of p53 pathway activation, transcriptional regulation mediated by TP53, and molecular mechanisms underlying tumor development. Through detection of endogenous p53 protein, clone CTA53-2R supports studies exploring genomic stability, oncogenic stress responses, and cellular signaling networks.
Beyond its classical tumor suppressor function, p53 participates in numerous biological processes including metabolic regulation, immune signaling, autophagy, and stem cell homeostasis. The protein interacts with regulatory partners such as MDM2, ATM, ATR, and transcriptional co-activators including p300 and CBP that influence transcriptional activity and protein stability. Because TP53 plays a central role in maintaining genomic integrity and preventing malignant transformation, reagents such as p53 Antibody Clone CTA53-2R remain valuable tools for investigating p53 signaling pathways and tumor biology.
Optimal dilution of the p53 Antibody Clone CTA53-2R / TP53 Tumor Suppressor Antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Recombinant human protein was used as the immunogen for this p53 Antibody Clone CTA53-2R / TP53 Tumor Suppressor Antibody.
Store the recombinant p53 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
TP53 antibody, Tumor protein p53 antibody, Cellular tumor antigen p53 antibody, p53 tumor suppressor antibody
Your bulk quote request has been submitted successfully!
Please contact us if you have any questions.
Powered by Bioz
Citations(12)