p40 Antibody for FACS / deltaNp63 Flow Cytometry Antibody [clone rTP40/3690] (V8627)

p40 Antibody. Immunohistochemistry analysis of Tumor protein p40 (deltaNp63) in FFPE human kidney tissue using a p40 antibody (clone rTP40/3690) demonstrates distinct HRP-DAB brown nuclear staining in epithelial cells lining tubular structures, while surrounding stromal and non-epithelial cells remain largely negative. The nuclear-restricted staining pattern is consistent with the localization of deltaNp63 as a transcription factor and highlights epithelial cell compartments within the kidney. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to antibody incubation.

IHC staining of FFPE human skin with recombinant p40 antibody (clone rTP40/3690). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

p40 Antibody for FACS. Flow cytometry analysis of PFA-fixed and permeabilized human HeLa cells using a p40 Antibody for FACS (clone rTP40/3690) demonstrates a clear rightward shift in fluorescence intensity for the deltaNp63-stained population (blue) compared to the isotype control (red). This separation indicates specific intracellular detection of Tumor protein p40 (deltaNp63) with minimal background signal. The distinct population shift supports accurate gating and quantification of p40-positive cells, consistent with nuclear expression of deltaNp63 following permeabilization. The recombinant mouse monoclonal format provides clean signal resolution and reproducible performance in flow cytometry-based detection of epithelial lineage-associated transcription factors.