p40 Antibody for IHC / deltaNp63 Immunohistochemistry Antibody [clone P40/7302R] (V9760)

p40 Antibody for IHC. Immunohistochemistry analysis of Tumor protein p40 (deltaNp63) in FFPE human prostate cancer tissue using a p40 Antibody for IHC (clone P40/7302R) demonstrates strong HRP-DAB brown nuclear staining in basal epithelial cells at the periphery of glandular structures, while malignant luminal tumor cells remain negative. The staining forms a continuous basal layer pattern in residual benign glands, clearly delineating glandular architecture and highlighting loss of basal cells in carcinoma regions. The nuclear-restricted signal with minimal background supports accurate interpretation of deltaNp63 expression and epithelial compartmentalization. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to antibody incubation.

p40 Antibody for IHC. Immunohistochemistry analysis of Tumor protein p40 (deltaNp63) in FFPE human prostate cancer tissue using a p40 Antibody for IHC (clone P40/7302R) demonstrates strong HRP-DAB brown nuclear staining in basal epithelial cells outlining benign glandular structures, while malignant luminal tumor cells remain negative. The staining pattern highlights a preserved basal cell layer in non-neoplastic glands and a clear loss of basal cells in carcinoma regions, supporting interpretation of epithelial architecture and tumor-associated changes. The nuclear-restricted signal shows minimal background, enabling confident identification of deltaNp63-positive cell populations. Inset: PBS was used in place of the primary antibody as a negative control, showing no brown signal and confirming specificity of the staining. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to antibody incubation.

p40 Antibody for IHC. Immunohistochemistry analysis of Tumor protein p40 (deltaNp63) in FFPE human ovarian cancer tissue using a p40 Antibody for IHC (clone P40/7302R) demonstrates focal HRP-DAB brown nuclear staining in tumor cell clusters, while surrounding stromal elements remain largely negative. The nuclear-restricted staining pattern supports detection of deltaNp63-positive tumor cells and highlights areas of epithelial differentiation within the tissue. Staining intensity and distribution vary across tumor regions, reflecting heterogeneity in p40 expression. Inset: PBS was used in place of the primary antibody as a negative control, showing no brown signal and confirming specificity of the staining. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to antibody incubation.