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Home >> Antibodies >> Recombinant p27Kip1 Antibody / p27

Recombinant p27Kip1 Antibody / p27 [clone rKIP1/1356] (V9161)

  Catalog No Formulation Size Price (USD)  
Image V9161-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9161-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9161SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC staining of FFPE human breast carcinoma tissue with recombinant p27Kip1 antibody (clone rKIP1/1356). Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant p27Kip1 antibody (clone rKIP1/1356) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rKIP1/1356
Purity Protein A/G affinity
UniProt P46527
Localization Nuclear
Applications Flow cytometry : 1-2ug/million cells
Immunofluorescence : 1-2ug/ml
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant p27Kip1 antibody is available for research use only.
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Description

p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF-beta-induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix.

Application Notes

Optimal dilution of the recombinant p27Kip1 antibody should be determined by the researcher.

Immunogen

Recombinant full-length human CDKN1B/p27Kip1 protein was used as the immunogen for the recombinant p27Kip1 antibody.

Storage

Aliquot the recombinant p27Kip1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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