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Home >> Antibodies >> Recombinant p27 Antibody / C-Terminal

Recombinant p27 Antibody / C-Terminal [clone RM302] (R20324)

  Catalog No Formulation Size Price (USD)  
Image R20324-0.1ML Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ul 419
Microvalidated Recrabbitmono
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IHC testing of formalin fixed and paraffin embedded human breast cancer tissue with recombinant p27 antibody at 1:200 dilution. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human MCF7 cell lysate with recombinant p27 antibody at 1:1000. Expected molecular weight: 22~27 kDa.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Mouse
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM302
Purity Protein A purified from animal origin-free supernatant
UniProt P46527
Localization Nuclear
Applications Immunohistochemistry (FFPE) : 1:100-1:200
Western Blot : 1:500-1:1000
Limitations This recombinant p27 antibody is available for research use only.
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Description

This antibody reacts to human p27Kip1 (Cyclin-dependent kinase inhibitor 1B). It may also react to mouse p27Kip1, as predicted by immunogen homology.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant p27 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A peptide corresponding to the C-terminus of human p27Kip1 was used as the immunogen for the recombinant p27 antibody.

Storage

Store the recombinant p27 antibody at -20oC.

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