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Home >> Antibodies >> Recombinant Nuclear Marker Antibody

Recombinant Nuclear Marker Antibody [clone NM2984R] (V7504)

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  Catalog No Formulation Size Price (USD)  
Image V7504-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7504-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7504SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V7504IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
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IHC staining of FFPE human tonsil with Nuclear Marker antibody (clone NM2984R). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool prior to testing.
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IHC staining of FFPE human tonsil with Nuclear Marker antibody (clone NM2984R). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool prior to testing.
IHC staining of FFPE human tonsil with Nuclear Marker antibody (clone NM2984R). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool prior to testing.
SDS-PAGE analysis of purified, BSA-free recombinant Nuclear Marker antibody (clone NM2984R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name NM2984R
Purity Protein G affinity chromatography
Localization Nuclei
Applications Immunohistochemistry (FFPE) : 0.25-0.5ug/ml for 30 min at RT
Limitations This recombinant Nuclear Marker antibody is available for research use only.
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Description

This MAb is an excellent marker for all nuclei in cells in tissues. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This MAb recognizes an antigen associated with the nuclei in all cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells.

Application Notes

Optimal dilution of the recombinant Nuclear Marker antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Nuclei of HL60 cells were used as the immunogen for the recombinant Nuclear Marker antibody.

Storage

Store the recombinant Nuclear Marker antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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