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Home >> Antibodies >> Recombinant NKX2.1 Antibody / TTF-1 Rabbit Monoclonal

Recombinant NKX2.1 Antibody / TTF-1 Rabbit Monoclonal [clone NX2.1/1855R] (V3655)

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  Catalog No Formulation Size Price (USD)  
Image V3655-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3655-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3655SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V3655IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
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IHC: Formalin-fixed, paraffin-embedded human thyroid stained with recombinant NKX2.1 antibody (clone NX2.1/1855R). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
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IHC: Formalin-fixed, paraffin-embedded human thyroid stained with recombinant NKX2.1 antibody (clone NX2.1/1855R). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC: Formalin-fixed, paraffin-embedded human lung adenocarcinoma stained with recombinant NKX2.1 antibody (clone NX2.1/1855R). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free recombinant NKX2.1 antibody (clone NX2.1/1855R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human, Mouse, and Rat
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name NX2.1/1855R
Purity Protein A affinity chromatography
UniProt P43699
Localization Nuclear
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This recombinant NKX2.1 antibody is available for research use only.
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Description

NKX2.1/TTF-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. Anti-NKX2.1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates. Loss of NKX2.1 expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. NKX2.1 reactivity is also seen in thyroid malignancies.

Application Notes

Optimal dilution of the recombinant NKX2.1 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant protein was used as the immunogen for the recombinant NKX2.1 antibody.

Storage

Store the recombinant NKX2.1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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   1.   View all NKX2.1 antibodies

   2.   View all recombinant antibodies

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