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Home >> Antibodies >> Recombinant NGFR Antibody / p75NTR

Recombinant NGFR Antibody / p75NTR [clone NGFR/2550R] (V7314)

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  Catalog No Formulation Size Price (USD)  
Image V7314-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7314-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7314SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V7314IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
Microvalidated Recrabbitmono
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IHC testing of FFPE human melanoma tissue with recombinant NGFR antibody (clone NGFR/2550R). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool prior to testing.
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IHC testing of FFPE human melanoma tissue with recombinant NGFR antibody. Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool prior to testing.
IHC testing of FFPE human melanoma tissue with recombinant NGFR antibody. Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool prior to testing.
SDS-PAGE analysis of purified, BSA-free recombinant NGFR antibody as confirmation of integrity and purity.
Western blot testing of human U-87 cell lysate with recombinant NGFR antibody (clone NGFR/2550R). Predicted molecular weight: 45-75 kDa depending on glycosylation level.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name NGFR/2550R
Purity Protein A affinity chromatography
UniProt P08138
Localization Cell surface and cytoplasmic
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Western blot : 1-2ug/ml
Limitations This recombinant NGFR antibody is available for research use only.
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Description

This antibody is specific for a glycoprotein of 75kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or p75 Neurotrophin Receptor (p75NTR). NGFR is expressed in various neural crest cells and their tumors such as melanocytes, melanomas, neuroblastomas, pheochromocytomas and neurofibromas. Reportedly, anti-NGFR/p75NTR is a reliable marker for desmoplastic and neurotropic melanomas. It is expressed in mature non-neural cells such as perivascular cells, dental pulp cells, lymphoidal follicular dendritic cells, basal epithelium of oral mucosa and hair follicles, prostate basal cells, and myoepithelial cells. Anti-NGFR/p75NTR stains the myoepithelial cells of breast ducts and intra-lobular fibroblasts of breast ducts.

Application Notes

Optimal dilution of the recombinant NGFR antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A portion of amino acids 281-421 from the human protein was used as the immunogen for the recombinant NGFR antibody.

Storage

Store the recombinant NGFR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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