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Home >> Antibodies >> Recombinant NGF Receptor Antibody / p75NTR / CD271

Recombinant NGF Receptor Antibody / p75NTR / CD271 [clone rNGFR/1965] (V7975)

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  Catalog No Formulation Size Price (USD)  
Image V7975-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7975-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7975SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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Western blot testing of human U-87 cell lysate with recombinant NGF Receptor antibody (clone rNGFR/1965). Predicted molecular weight: 45-75 kDa depending on glycosylation level.
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Western blot testing of human U-87 cell lysate with recombinant NGF Receptor antibody (clone rNGFR/1965). Predicted molecular weight: 45-75 kDa depending on glycosylation level.
IHC staining of FFPE human melanoma with recombinant NGF Receptor antibody (clone rNGFR/1965). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human melanoma with recombinant NGF Receptor antibody (clone rNGFR/1965). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant NGF Receptor antibody (clone rNGFR/1965) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rNGFR/1965
Purity Protein G affinity chromatography
UniProt P08138
Localization Cell surface, cytoplasmic
Applications Immunohistochemistry (FFPE) : 1-2ug/ml (not suitable for frozen sample testing)
Western blot : 1-2ug/ml
Limitations This recombinant NGF Receptor antibody is available for research use only.
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Description

It recognizes a glycoprotein of 75kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). NGFR is expressed in various neural crest cells and their tumors such as melanocytes, melanomas, neuroblastomas, pheochromocytomas and neurofibromas. Reportedly, anti-NGFR is a reliable marker for desmoplastic and neurotropic melanomas. NGFR is expressed in mature non-neural cells such as perivascular cells, dental pulp cells, lymphoidal follicular dendritic cells, basal epithelium of oral mucosa and hair follicles, prostate basal cells, and myoepithelial cells. Anti-NGFR stains the myoepithelial cells of breast ducts and intra-lobular fibroblasts of breast ducts.

Application Notes

Optimal dilution of the recombinant NGF Receptor antibody should be determined by the researcher.

Immunogen

A recombinant human partial protein (amino acids 281-421) was used as the immunogen for this recombinant NGF Receptor antibody.

Storage

Store the recombinant NGF Receptor antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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