NGF Receptor Antibody rNGFR/1965 / Low Affinity NGF Receptor Antibody [clone rNGFR/1965] (V7975)

NGF Receptor Antibody rNGFR/1965 by WB in Human U-87 Cell Lysate. Western blot analysis of human U-87 glioblastoma cell lysate using recombinant mouse monoclonal NGF Receptor antibody rNGFR/1965. A distinct immunoreactive band is observed at approximately 60 kDa, within the reported 45-75 kDa molecular weight range for p75NTR (CD271). Differences in glycosylation and protein processing can influence the electrophoretic migration of this transmembrane receptor. The blot shows one predominant band with minimal additional immunoreactivity, supporting detection of NGFR in human U-87 cells. The observed migration is consistent with the expected molecular weight range of the low affinity NGF receptor, also known as TNFRSF16.

NGF Receptor Antibody rNGFR/1965 by IHC in Human Melanoma. Immunohistochemistry staining of FFPE human melanoma tissue using recombinant mouse monoclonal NGF Receptor antibody rNGFR/1965. Strong brown immunoreactivity is observed in numerous tumor cells, with predominantly membranous and peripheral cytoplasmic staining. Positive cells are distributed throughout the examined tissue, although staining intensity varies between individual cells. The pattern is consistent with expression of the low affinity NGF receptor, also known as p75NTR and CD271. Heat-induced epitope retrieval was performed using 10 mM Tris with 1 mM EDTA, pH 9, for 20 minutes, followed by cooling before antibody incubation.

NGF Receptor Antibody rNGFR/1965 by IHC Showing Variable Staining in Human Melanoma. Immunohistochemistry staining of FFPE human melanoma tissue using recombinant mouse monoclonal NGF Receptor antibody rNGFR/1965. Prominent brown staining is visible in irregular groups of tumor cells, with a predominantly membranous and peripheral cytoplasmic distribution. Staining intensity varies across the specimen, with strongly positive cells interspersed among more weakly reactive areas. This heterogeneous pattern illustrates the distribution of p75NTR (CD271) within melanoma tissue. Heat-induced epitope retrieval was performed using 10 mM Tris with 1 mM EDTA, pH 9, for 20 minutes, followed by cooling before antibody incubation.

NGF Receptor Antibody rNGFR/1965 by SDS-PAGE Under Reducing and Non-Reducing Conditions. SDS-PAGE analysis of purified, BSA-free recombinant mouse monoclonal NGF Receptor antibody rNGFR/1965, with 2.5 ug of antibody loaded per lane. Under non-reducing (NR) conditions, the predominant band migrates near 150 kDa, consistent with intact IgG. Under reducing (R) conditions, heavy and light chains are observed at approximately 50 kDa and 25 kDa, respectively. These results support the structural integrity and purity of the recombinant antibody used to recognize p75NTR, also known as CD271 and TNFRSF16.